| Title: | Volatile organic compounds generated by cultures of bacteria and viruses associated with respiratory infections |
| Author(s): | Abd El Qader A; Lieberman D; Shemer Avni Y; Svobodin N; Lazarovitch T; Sagi O; Zeiri Y; |
| Address: | "Department of Biomedical Engineering, Ben-Gurion University of the Negev, Beer-Sheva, Israel. Pulmonary Unit, Soroka University Medical Center and the Faculty of Health, Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel. The Laboratory of Viral Diagnostics, Soroka University Medical Center, Faculty of Health Sciences, Ben Gurion University of the Negev, Beer-Sheva, Israel. Department of Clinical Microbiology, Assaf Harofeh Medical Center, Zerifin, Israel. Parasitology Laboratory, Soroka University Medical Center, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel. Division of Chemistry, Nuclear Research Center, Negev, Beer-Sheva, Israel" |
| ISSN/ISBN: | 1099-0801 (Electronic) 0269-3879 (Linking) |
| Abstract: | "Respiratory infections (RI) can be viral or bacterial in origin. In either case, the invasion of the pathogen results in production and release of various volatile organic compounds (VOCs). The present study examines the VOCs released from cultures of five viruses (influenza A, influenza B, adenovirus, respiratory syncitial virus and parainfluenza 1 virus), three bacteria (Moraxella catarrhalis, Haemophilus influenzae and Legionella pneumophila) and Mycoplasma pneumoniae isolated colonies. Our results demonstrate the involvement of inflammation-induced VOCs. Two significant VOCs were identified as associated with infectious bacterial activity, heptane and methylcyclohexane. These two VOCs have been linked in previous studies to oxidative stress effects. In order to distinguish between bacterial and viral positive cultures, we performed principal component analysis including peak identity (retention time) and VOC concentration (i.e. area under the peak) revealing 1-hexanol and 1-heptadecene to be good predictors" |
| Keywords: | Bacteria/*metabolism Bacterial Infections/microbiology Biomarkers/*analysis/metabolism Cyclohexanes/analysis/metabolism Gas Chromatography-Mass Spectrometry/methods Heptanes/analysis/metabolism Humans *Respiratory Tract Infections/microbiology/virology Vi; |
| Notes: | "MedlineAbd El Qader, Amir Lieberman, David Shemer Avni, Yonat Svobodin, Natali Lazarovitch, Tsilia Sagi, Orli Zeiri, Yehuda eng England 2015/06/03 Biomed Chromatogr. 2015 Dec; 29(12):1783-90. doi: 10.1002/bmc.3494. Epub 2015 May 27" |
