| Title: | Putative regulatory role of hexokinase and fructokinase in terpenoid aroma biosynthesis in Lilium 'Siberia' |
| Author(s): | Abbas F; Nian X; Zhou Y; Ke Y; Liu L; Yu R; Fan Y; |
| Address: | "The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China. College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China. College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China. Electronic address: rcyu@scau.edu.cn. The Research Center for Ornamental Plants, College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, 510642, China; Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm, South China Agricultural University, Guangzhou, 510642, China. Electronic address: fanyanping@scau.edu.cn" |
| DOI: | 10.1016/j.plaphy.2021.08.042 |
| ISSN/ISBN: | 1873-2690 (Electronic) 0981-9428 (Linking) |
| Abstract: | "Lily is one of the most economically important flowers worldwide due to its elegant appearance and appealing scent, which is mainly composed of monoterpene ocimene, linalool and benzenoids. Sugars are the primary products of plants, with fructose and hexose sugars being the substrate material for most organic compounds and metabolic pathways in plants. Herein, we isolated and functionally characterized hexokinase (LoHXK) and fructokinase (LoFRK) from Lilium 'Siberia' flower, which indicated their potential roles in floral aroma production. Real-time PCR analysis showed that LoHXK and LoFRK were highly expressed in the flower filament. Overexpression and virus-induced gene silencing (VIGS) assays revealed that LoHXK and LoFRK significantly modified the emission of beta-ocimene and linalool contents via regulation of expression of key structural volatile synthesis genes (LoTPS1 and LoTPS3). Under exogenous glucose and fructose application, the volatile contents of beta-ocimene and linalool were increased and the expression levels of key structural genes were upregulated. The emission of beta-ocimene and linalool followed a diurnal circadian rhythm. Determination of carbon fluxes via (13)C-labeled glucose and (13)C-labeled fructose experiments showed that the mass spectra of ocimene and linalool significantly increased, however, the m/z ratio of ethyl benzoate did not change. Furthermore, yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays showed that LoFRK interacted with LoMYB1 and LoMYB2 proteins. Together, these results suggest that hexokinase and fructokinase may play significant roles in the regulation of ocimene and linalool biosynthesis in Lilium 'Siberia'" |
| Keywords: | "Flowers/enzymology *Fructokinases/genetics Gene Expression Regulation, Plant *Hexokinase/genetics *Lilium/enzymology/genetics *Odorants Floral scent Fructokinase Hexokinase Lilium 'Siberia' Terpene synthase;" |
| Notes: | "MedlineAbbas, Farhat Nian, Xinxin Zhou, Yiwei Ke, Yanguo Liu, Liang Yu, Rangcai Fan, Yanping eng France 2021/09/04 Plant Physiol Biochem. 2021 Oct; 167:619-629. doi: 10.1016/j.plaphy.2021.08.042. Epub 2021 Aug 30" |
