| Title: | Investigation of bacterial viability from incubated saliva by application of flow cytometry and hyphenated separation techniques |
| Author(s): | Buszewski B; Milanowski M; Ligor T; Pomastowski P; |
| Address: | "Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Torun, Poland. Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, Torun, Poland" |
| ISSN/ISBN: | 1522-2683 (Electronic) 0173-0835 (Linking) |
| Abstract: | "The aim of the study was determination of bacterial viability in saliva samples and finding a correlation between microbiological and volatile profiles of saliva depending on incubation time. Bacteria colonizing healthy oral cavities were also identified. Twelve healthy adults donated unstimulated saliva samples. Flow cytometry, optical density measurements and colony-forming unit (CFU) counting method were employed for analyses of native and inoculated saliva after 0, 1, 2, 24, and 48 h of incubation. Volatile profiles were acquired using headspace-solid phase microextraction-gas chromatography/mass spectrometry (HS-SPME-GC/MS). Oral bacteria were the most viable within 2 h after collection of saliva. Extension of incubation time to 48 h caused considerable decrease in live bacteria counts and sharp increase in dead bacteria counts. The most prevalent strain was Sphingomonas paucimobilis (26.67%). The number of volatiles raised from 5 to 27 with incubation time and most of them were putrefaction products, such as methanethiol, indole and pyrrole. HS-SPME-GC/MS method is insufficient for volatile profiling of 'fresh' saliva and should be directed rather to investigation of bacterial metabolites" |
| Keywords: | Adult Bacteria/*isolation & purification Cell Culture Techniques Flow Cytometry Gas Chromatography-Mass Spectrometry Humans Metabolome *Microbial Viability Saliva/*microbiology Solid Phase Microextraction Volatile Organic Compounds/*analysis Bacteria Hs-s; |
| Notes: | "MedlineBuszewski, Boguslaw Milanowski, Maciej Ligor, Tomasz Pomastowski, Pawel eng Germany 2017/04/22 Electrophoresis. 2017 Aug; 38(16):2081-2088. doi: 10.1002/elps.201700057. Epub 2017 May 15" |
