| Title: | Effect of muscone on anti-apoptotic ability of muskrat prostate primary cells |
| Author(s): | Zhang Y; Zhao X; Man X; Ma Y; Zhang W; Wang Y; Lei X; Bai S; |
| Address: | "College of Wildlife and Protected Area, Northeast Forestry University, Harbin, People's Republic of China. National and Local Joint Engineering Laboratory for Freshwater Fish Breeding, Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin, People's Republic of China. Detecting Center of Wildlife, State Forestry and Grassland Administration, Harbin, People's Republic of China" |
| Journal Title: | Am J Physiol Regul Integr Comp Physiol |
| DOI: | 10.1152/ajpregu.00068.2022 |
| ISSN/ISBN: | 1522-1490 (Electronic) 0363-6119 (Print) 0363-6119 (Linking) |
| Abstract: | "Muskrat is a small fur animal with a pair of scent glands that can secrete muskrat musk during breeding season. The consensus is muskrat musk functions as a pheromone, but we hypothesized it has a broader role. In previous research, we found the presence of muscone in muskrat musk. To study whether the muscone can affect the apoptosis of muskrat prostate, we carried out the following investigations. Primary muskrat prostate cells were cultured and treated with muscone. Then we drew cell proliferation curves by applying the CCK-8 and used TdT-mediated dUTP nick end labeling (TUNEL) to detect apoptosis. Levels of mRNA transcription and protein expression of Bcl-2 as well as Bax were detected by qRT-PCR and the Western blot. Meanwhile, we collected tissue samples of muskrat prostates and froze sections to analyze the fluorescence signal intensity of BCL-2 and BAX via immunofluorescence. Under the treatment of 30 mumol/L muscone, the proliferation rate of the experimental group exceeded that of the control group, and the proportion of cells undergoing apoptosis was lower in the experimental group. The qRT-PCR and Western blot result showed that, in the experimental group, the ratio of Bcl-2 to Bax mRNA transcription levels increased by 2.85 times and their corresponding protein expression ratio increased by 2.37 times (P < 0.05). Immunofluorescence results were consistent with the cell experiment's results. The fluorescence signal intensity of BCL-2 was higher in the breeding season than nonbreeding season but vice versa for BAX. Based on these results, we speculate that the muscone could regulates prostate development by inhibiting apoptosis" |
| Keywords: | "Animals Apoptosis *Arvicolinae/physiology Cycloparaffins Male Pheromones/metabolism *Prostate/metabolism Proto-Oncogene Proteins c-bcl-2/genetics/metabolism RNA, Messenger/metabolism Sincalide/metabolism bcl-2-Associated X Protein/genetics/metabolism musc;" |
| Notes: | "MedlineZhang, Yu Zhao, Xuefei Man, Xin Ma, Yue Zhang, Wei Wang, Yu Lei, Xing Bai, Suying eng Research Support, Non-U.S. Gov't 2022/08/16 Am J Physiol Regul Integr Comp Physiol. 2022 Oct 1; 323(4):R571-R580. doi: 10.1152/ajpregu.00068.2022. Epub 2022 Aug 15" |
