| Title: | Identification and functional characterization of sex pheromone receptors in mirid bugs (Heteroptera: Miridae) |
| Author(s): | Zhang S; Yan S; Zhang Z; Cao S; Li B; Liu Y; Wang G; |
| Address: | "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China. Electronic address: yangliu@ippcaas.cn. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China; Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, China. Electronic address: wangguirong@caas.cn" |
| DOI: | 10.1016/j.ibmb.2021.103621 |
| ISSN/ISBN: | 1879-0240 (Electronic) 0965-1748 (Linking) |
| Abstract: | "Mirid bugs are a group of important insect pests that cause large annual losses in agricultural production. Many studies have focused on the isolation and identification of sex pheromones in mirid bugs, and the components and biological activity of the sex pheromones have also been studied as a way to control these pests. However, few studies have focused on the mechanisms of pheromone perception. In this study, we identified the odorant receptor repertoire in three mirid bug species, Apolygus lucorum, Adelphocoris lineolatus, and Adelphocoris suturalis using antennal transcriptome sequencing and bioinformatics analysis. The candidate pheromone receptor (PR) genes were then identified by comparative transcriptomic and expression pattern analysis. Importantly, in vitro functional studies have shown that the candidate PRs have robust responses to the main mirid bug sex pheromone components (E)-2-hexenyl butyrate (E2HB) and hexyl butyrate (HB). Our study uncovered the mechanism of pheromone peripheral coding in these three species and elucidated the mechanism by which mirid bugs can specifically recognize a mate. Moreover, the results of our study will provide a theoretical basis for screening effective sex attractants or mating disturbance agents at the molecular and neural levels for enhanced control of these destructive pests" |
| Keywords: | "Animals Gene Expression Profiling Genes, Insect *Heteroptera/genetics/metabolism Pest Control/methods Receptors, Odorant/chemistry/genetics/metabolism *Receptors, Pheromone/chemistry/genetics/metabolism Sex Attractants/metabolism Sexual Behavior, Animal E;" |
| Notes: | "MedlineZhang, Sai Yan, Shuwei Zhang, Zhixiang Cao, Song Li, Bin Liu, Yang Wang, Guirong eng Research Support, Non-U.S. Gov't England 2021/07/08 Insect Biochem Mol Biol. 2021 Sep; 136:103621. doi: 10.1016/j.ibmb.2021.103621. Epub 2021 Jul 4" |
