| Title: | Isolation and characterization of antagonistic Paenibacillus polymyxa HX-140 and its biocontrol potential against Fusarium wilt of cucumber seedlings |
| Author(s): | Zhai Y; Zhu JX; Tan TM; Xu JP; Shen AR; Yang XB; Li JL; Zeng LB; Wei L; |
| Address: | "Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, No.348 Xianjiahu West Road, Changsha, 410205, Hunan, China. Hunan Provincial Key Laboratory of Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, 410004, China. Hunan Academy of Forestry, Changsha, 410004, China. Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, No.348 Xianjiahu West Road, Changsha, 410205, Hunan, China. zengliangbin@caas.cn. Institute of Plant Protection, Hunan Academy of Agricultural Sciences, No.726 Yuanda 2nd Road, Changsha, 410125, Hunan, China. nkyweilin@163.com" |
| DOI: | 10.1186/s12866-021-02131-3 |
| ISSN/ISBN: | 1471-2180 (Electronic) 1471-2180 (Linking) |
| Abstract: | "OBJECTIVE: The aim of this study is to evaluate the efficacy of the strain Paenibacillus polymyxa HX-140, isolated from the rhizosphere soil of rape, to control Fusarium wilt of cucumber seedlings caused by Fusarium oxysporum f. sp. cucumerinum. RESULTS: Strain HX-140 was able to produce protease, cellulase, beta-1,3-glucanase and antifungal volatile organic compounds. An in vitro dual culture test showed that strain HX-140 exhibited broad spectrum antifungal activity against soil-borne plant pathogenic fungi. Strain HX-140 also reduced the infection of Fusarium wilt of cucumber seedlings by 55.6% in a greenhouse pot experiment. A field plot experiment confirmed the biocontrol effects and further revealed that antifungal activity was positively correlated with inoculum size by the root-irrigation method. Here, inoculums at 10(6) 10(7) and 10(8) cfu/mL of HX-140 bacterial suspension reduced the incidence of Fusarium wilt of cucumber seedling by 19.5, 41.1, and 50.9%, respectively. CONCLUSIONS: Taken together, our results suggest that P. polymyxa HX-140 has significant potential in the control of Fusarium wilt and possibly other fungal diseases of cucumber" |
| Keywords: | *Biological Control Agents Brassica napus/microbiology Cucumis sativus/*microbiology Fusarium/*physiology Microbial Interactions/*physiology Paenibacillus polymyxa/*physiology Plant Diseases/microbiology/*prevention & control Seedlings/microbiology Soil M; |
| Notes: | "MedlineZhai, Yang Zhu, Jiu-Xiang Tan, Tai-Meng Xu, Jian-Ping Shen, Ai-Rong Yang, Xie-Bin Li, Ji-Lie Zeng, Liang-Bin Wei, Lin eng Research Support, Non-U.S. Gov't England 2021/03/08 BMC Microbiol. 2021 Mar 6; 21(1):75. doi: 10.1186/s12866-021-02131-3" |
