« Previous AbstractEffects of Three Volatile Oxylipins on Colony Development in Two Species of Fungi and on Drosophila Larval Metamorphosis    Next AbstractGenomic Analyses of Penicillium Species Have Revealed Patulin and Citrinin Gene Clusters and Novel Loci Involved in Oxylipin Production »

J Ind Microbiol Biotechnol

Title:		Influence of R and S enantiomers of 1-octen-3-ol on gene expression of Penicillium chrysogenum
Author(s):		Yin G; Zhang Y; Fu M; Hua SST; Huang Q; Pennerman KK; Wu G; Jurick WM; Lee S; Bu L; Zhao H; Bennett JW;
Address:		"Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, China. guohuayin1997@gmail.com. Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, NJ, 08901, USA. guohuayin1997@gmail.com. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, China. Department of Agriculture, ARS, Western Regional Research Center, Albany, CA, 94710, USA. Department of Plant Biology and Pathology, Rutgers, The State University of New Jersey, New Brunswick, NJ, 08901, USA. Department of Energy, Oak Ridge Institute for Science and Education, Oak Ridge, TN, 37831, USA. Department of Agriculture, Food Quality Laboratory, ARS, Beltsville Agricultural Research Center, Beltsville, MD, 20705, USA. Department of Biology, Center for Evolutionary and Theoretical Immunology CETI, University of New Mexico, Albuquerque, NM, 87131, USA. Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, Hainan, China. zhaohui@itbb.org.cn"
Journal Title:		J Ind Microbiol Biotechnol
Year:		2019
Volume:		20190328
Issue:		7
Page Number:		977 - 991
DOI:		10.1007/s10295-019-02168-4
ISSN/ISBN:		1476-5535 (Electronic) 1367-5435 (Linking)
Abstract:		"Inhibition of spore germination offers an attractive and effective target for controlling fungal species involved in food spoilage. Mushroom alcohol (1-octen-3-ol) functions as a natural self-inhibitor of spore germination for many fungi and, therefore, provides a useful tool for probing the molecular events controlling the early stages of fungal growth. In Penicillium spp., the R and S enantiomers of 1-octen-3-ol delayed spore germination and sporulation in four species of Penicillium involved in soils of fruit and grains, but to different degrees. Because of its well-annotated genome, we used Penicillium chrysogenum to perform a comprehensive comparative transcriptomic analysis of cultures treated with the two enantiomers. Altogether, about 80% of the high-quality reads could be mapped to 11,396 genes in the reference genome. The top three active pathways were metabolic (978 transcripts), biosynthesis of secondary metabolites (420 transcripts), and microbial metabolism in diverse environments (318 transcripts). When compared to the control, treatment with (R)-(-)-1-octen-3-ol affected the transcription levels of 91 genes, while (S)-(+)-1-octen-3-ol affected only 41 genes. Most of the affected transcripts were annotated and predicted to be involved in transport, establishment of localization, and transmembrane transport. Alternative splicing and SNPs' analyses indicated that, compared to the control, the R enantiomer had greater effects on the gene expression pattern of Penicillium chrysogenum than the S enantiomer. A qRT-PCR analysis of 28 randomly selected differentially expressed genes confirmed the transcriptome data. The transcriptomic data have been deposited in NCBI SRA under the accession number SRX1065226"
Keywords:		Gene Expression Octanols/chemistry/*metabolism Penicillium/drug effects Penicillium chrysogenum/genetics/*metabolism Stereoisomerism Transcriptome (R)-(-)-1-octen-3-ol (S)-(+)-1-octen-3-ol Fungal storage contaminants Penicillium chrysogenum Transcriptome;
Notes:		"MedlineYin, Guohua Zhang, Yuliang Fu, Maojie Hua, Sui Sheng T Huang, Qixing Pennerman, Kayla K Wu, Guangxi Jurick, Wayne M 2nd Lee, Samantha Bu, Lijing Zhao, Hui Bennett, Joan W eng 201403075/he Special Fund for Agro-scientific Research in the Public Interest of the People's Republic of China/ 2-47012/the USDA-ARS Cooperative Agreement/ Germany 2019/03/30 J Ind Microbiol Biotechnol. 2019 Jul; 46(7):977-991. doi: 10.1007/s10295-019-02168-4. Epub 2019 Mar 28"