| Title: | Functional characterization of chemosensory proteins in response to artificial light treatment in Thalassodes immissaria |
| Author(s): | Yao Q; Liang Z; Quan L; Chen B; |
| Address: | "Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Institute of Plant Protection, Guangdong Academy of Agricultural Sciences, Guangzhou, China. School of Life Sciences, South China Normal University, Guangzhou, China" |
| ISSN/ISBN: | 1526-4998 (Electronic) 1526-498X (Linking) |
| Abstract: | "BACKGROUND: Chemosensory proteins (CSPs) play a vital role in the response to environmental stimuli in insects. However, the involvement of insect CSPs in the stress response to night-time environmental changes has not been examined. RESULTS: In the current study, four TiCSP genes were first cloned from Thalassodes immissaria by transcriptome and RACE-PCR techniques. TiCSPs had typical characteristics of insect CSPs, including a highly conserved four-cysteine motif and olfactory-specific protein D (OS-D) or OS-D superfamily domains. TiCSP1-4 were clustered classified within different clades in a phylogenetic analysis and were differentially expressed at all developmental stages. Under night-time artificial light stress, the expression levels of TiCSP1 in males were significantly decreased at 24 h, and those of TiCSP2 were decreased in both adult sexes at 48 h. In a molecular docking analysis, TiCSPs showed relatively higher binding affinities with sex pheromone components than with host plant volatile molecules. CONCLUSION: Taking the reduced expression levels of TiCSPs and binding affinities into account, TiCSP1 and TiCSP2 are involved in the stress response processes of T. immissaria under light treatment. Our study supplies basic data for the evaluation of the effects of light interference control technology - an emerging physical control measure on nontarget pests of lychee orchards. (c) 2023 Society of Chemical Industry" |
| Keywords: | "Animals Male Gene Expression Profiling Phylogeny Molecular Docking Simulation Transcriptome Insecta/genetics *Lepidoptera/genetics Insect Proteins/metabolism *Receptors, Odorant/chemistry Arthropod Antennae Thalassodes immissaria artificial light at night;" |
| Notes: | "MedlineYao, Qiong Liang, Zhantu Quan, Linfa Chen, Bingxu eng CARS-32/Earmarked Fund for Litchi and Longan/ XTXM202202/Funds of GDAAS/ 32102222/National Natural Science Foundation of China/ 31801800/National Natural Science Foundation of China/ England 2023/01/10 Pest Manag Sci. 2023 May; 79(5):1760-1767. doi: 10.1002/ps.7351. Epub 2023 Jan 16" |
