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J Chem Ecol

Title:		"Molecular characterization of two pheromone binding proteins and quantitative analysis of their expression in the beet armyworm, Spodoptera exigua Hubner"
Author(s):		Xiu WM; Dong SL;
Address:		"Key Laboratory of Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China"
Journal Title:		J Chem Ecol
Year:		2007
Volume:		20070329
Issue:		5
Page Number:		947 - 961
DOI:		10.1007/s10886-007-9277-2
ISSN/ISBN:		0098-0331 (Print) 0098-0331 (Linking)
Abstract:		"Pheromone binding proteins (PBP) play an important role in insect pheromone communication. However, the PBP for the beet armyworm, Spodoptera exigua Hubner (Lepidoptera: Noctuidae), an important agricultural pest worldwide, remains unaddressed. We report the cloning of two PBP genes, SexigPBP1 and SexigPBP2, from the antennal cDNA of S. exigua by reverse transcriptase-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends-PCR (RACE-PCR). The deduced PBP amino acid sequences are characteristic of the odorant binding protein (OBP) family, although the two PBPs are only 44% identical. From an analysis of the genomic DNA, two introns and a similar intron/extron structural pattern were identified in each of the two PBP genes. RT-PCR analysis revealed that the two PBP genes are only expressed in antennae. Real-time PCR further indicated that the expression of SexigPBP1 is much higher than that of SexigPBP2, regardless of sex. However, the female expression levels for SexigPBP1 and SexigPBP2 are about 39% and 73%, respectively, relative to male levels. Finally, phylogenetic analysis suggested that PBPs from the Noctuidae are divided into three distinct groups based on the primary sequences"
Keywords:		"Amino Acid Sequence Animals Base Sequence Carrier Proteins/*genetics DNA, Complementary/genetics Female Gene Expression Insect Proteins/*genetics Male Molecular Sequence Data Phylogeny RNA, Messenger/metabolism Sequence Analysis, DNA Sequence Homology, Am;"
Notes:		"MedlineXiu, Wei-Ming Dong, Shuang-Lin eng Research Support, Non-U.S. Gov't 2007/03/30 J Chem Ecol. 2007 May; 33(5):947-61. doi: 10.1007/s10886-007-9277-2. Epub 2007 Mar 29"