| Title: | Dietary Exposure to Oxidized Frying Oil from Fetus to Adulthood Suppresses Male Reproductive Development by Altering Testicular Cholesterol and Testosterone Homeostasis in Sprague Dawley Rats |
| Author(s): | Wu HP; Lin YS; Chang CF; Lu SY; Chao PM; |
| Address: | "Department of Nutrition, China Medical University, Taichung, Taiwan. Department of Anatomy, School of Medicine, China Medical University, Taichung, Taiwan. Department of Applied Toxicology, Taiwan Agricultural Chemicals and Toxic Substances Research Institute, Taichung, Taiwan" |
| ISSN/ISBN: | 1541-6100 (Electronic) 0022-3166 (Linking) |
| Abstract: | "BACKGROUND: Dietary frying oil may have endocrine-disrupting effects, as a feminization effect was observed in cohorts of C57BL/6J male mice fetuses from dams consuming oxidized frying oil (OFO) during pregnancy. OBJECTIVE: The aim of present study was to test the hypothesis that OFO is an anti-androgen. METHODS: In experiment 1, male progeny of Sprague Dawley female rats fed fresh oil or an OFO diet (10 g fat/100 g, from fresh or 24-h-fried soybean oil; [control diet (C) and OFO groups, respectively] from midgestation through lactation were studied. Pups were weaned at 3 wk of age and then consumed their mothers' diet until 9 wk of age. In addition, a group of dams and pups that consumed a high-fat diet (HF; 10 g fried and 20 g fresh soybean oil/100 g) was included to counteract body-weight loss associated with OFO ingestion. Indices of male reproductive development and testosterone homeostasis were measured. In experiment 2, male rats were allocated to C and OFO groups (treated as above) and indices of male fertility compared at 9-10 wk of age. RESULTS: In experiment 1, final body weights of the HF group were lower (17%) than the C group but higher (14%) than the OFO group (P < 0.0001 for each). In addition to abnormalities in seminiferous tubules, HF and OFO groups did not differ from one another, but, compared with the C group, had delayed preputial separation (4.9 d) and reductions in serum testosterone concentrations (17-74%), anogenital distance (8-20%), weights of androgen-dependent tissues (8-30%), testicular testosterone and cholesterol concentrations (30-40%), and mRNA levels of genes involved in steroidogenesis and cholesterol homeostasis (30-70%). In experiment 2, OFO-exposed males had 20% lower sperm motility (P < 0.05); however, when mated to normal females, pregnancy rates and litter sizes did not differ between OFO and C groups. CONCLUSIONS: The anti-androgenic effect of OFO in Sprague Dawley rats was attributed to decreased testicular concentrations of cholesterol (testosterone precursor) and not body-weight loss" |
| Keywords: | Animal Nutritional Physiological Phenomena Animals Cholesterol/*metabolism Cooking Dietary Fats/administration & dosage/toxicity Female Homeostasis/*drug effects Male Oxidation-Reduction Pregnancy Prenatal Exposure Delayed Effects Prenatal Nutritional Phy; |
| Notes: | "MedlineWu, Hai-Ping Lin, Yu-Shun Chang, Chi-Fen Lu, Shui-Yuan Chao, Pei-Min eng Research Support, Non-U.S. Gov't 2020/04/15 J Nutr. 2020 Jul 1; 150(7):1713-1721. doi: 10.1093/jn/nxaa091" |
