« Previous AbstractComplex nature of enterococcal pheromone-responsive plasmids    Next AbstractA Miniature Gas Sampling Interface with Open Microfluidic Channels: Characterization of Gas-to-Liquid Extraction Efficiency of Volatile Organic Compounds »

Plasmid

Title:		Abundance and diversity of plasmid-associated genes among clinical isolates of Enterococcus faecalis
Author(s):		Wardal E; Gawryszewska I; Hryniewicz W; Sadowy E;
Address:		"National Medicines Institute, ul. Chelmska 30/34, 00-725 Warsaw, Poland. Electronic address: ewardal@cls.edu.pl"
Journal Title:		Plasmid
Year:		2013
Volume:		20130729
Issue:		3
Page Number:		329 - 342
DOI:		10.1016/j.plasmid.2013.07.003
ISSN/ISBN:		1095-9890 (Electronic) 0147-619X (Linking)
Abstract:		"Enterococcus faecalis, a normal compound of the human intestinal microbiome, plays an important role in hospital-acquired infections. Plasmids make a significant contribution to the acquisition of the novel traits such as antimicrobial resistance and virulence by this pathogen. The study investigated the plasmid content and the diversity of plasmid-associated genes in a group of 152 hospital isolates of E. faecalis. The majority of plasmids visualized by pulsed-field gel electrophoresis of S1 nuclease-digested DNA fell into the range of 50-100 kb. PCR-based screening allowed detection of genes of the rep1(pIP501), rep2(pRE25), rep4(pMBB1), rep6(pS86), rep7(pT181), rep8(pAM373), rep9(pAD1/pTEF2/pCF10), rep10(pIM13) and rep13(pC194) families in 29 different combinations. The par and omega-epsilon-zeta plasmid stabilization systems were ubiquitous (45 isolates, 29.6% and 88 isolates, 57.9%, respectively), while the axe-txe system was not found. The asa1 gene homologues encoding aggregation substance characteristic for the pAD1 and related group of pheromone-responsive plasmids were present in 106 isolates. A variety of sequence variants, including novel ones, of genes associated with pheromone-responsive plasmids, such as rep8(pAM373), rep9(pAD1/pTEF2/pCF10), par, and asa1 were observed. In conclusion, there is a big and only partially characterized pool of diverse plasmids in clinical E. faecalis"
Keywords:		"Bacterial Proteins/classification/*genetics DNA Transposable Elements Deoxyribonucleases/metabolism Electrophoresis, Gel, Pulsed-Field Enterococcus faecalis/classification/*genetics/isolation & purification *Gene Expression Regulation, Bacterial *Genes, B;"
Notes:		"MedlineWardal, Ewa Gawryszewska, Iwona Hryniewicz, Waleria Sadowy, Ewa eng Research Support, Non-U.S. Gov't 2013/08/03 Plasmid. 2013 Nov; 70(3):329-42. doi: 10.1016/j.plasmid.2013.07.003. Epub 2013 Jul 29"