| Title: | Newly isolated Enterobacter cloacae sp. HN01 and Klebsiella pneumoniae sp. HN02 collaborate with self-secreted biosurfactant to improve solubility and bioavailability for the biodegradation of hydrophobic and toxic gaseous para-xylene |
| Author(s): | Wang Y; Wan S; Yu W; Yuan D; Sun L; |
| Address: | "School of Chemical Engineering and Technology, Hainan University, Haikou, 570228, China. School of Chemical Engineering and Technology, Hainan University, Haikou, 570228, China; Key Laboratory of Advanced Materials of Tropical Island Resources, Ministry of Education, Haikou, 570228, China. College of Ecology and Environment, Hainan University, Haikou, 570228, China. School of Chemical Engineering and Technology, Hainan University, Haikou, 570228, China; Key Laboratory of Advanced Materials of Tropical Island Resources, Ministry of Education, Haikou, 570228, China. Electronic address: sunlei0620@163.com" |
| DOI: | 10.1016/j.chemosphere.2022.135328 |
| ISSN/ISBN: | 1879-1298 (Electronic) 0045-6535 (Linking) |
| Abstract: | "The gas-liquid mass transfer rate of hydrophobic volatile organic compounds (VOCs) is the limiting step in a biological treatment system. The present study aimed to utilize self-producing biosurfactants to enhance the bioavailability of hydrophobic gaseous VOCs. Two novel gram-negative rod-shaped bacteria, Enterobacter cloacae strain HN01 and Klebsiella pneumoniae strain HN02 were successfully isolated from sewage sludge by using blood agar and methylene blue agar plates. The two strains can use para-xylene (PX), a hydrophobic VOC model, as the only carbon source for biosurfactant production. Both strains can produce glycolipid biosurfactants, as confirmed by the emulsification index, Nuclear magnetic resonance, and Fourier transform infrared spectroscopy. Results indicated that PX can be completely decomposed at an initial concentration of 15.50 mg L(-1), pH value of 7.0, and temperature of 30 degrees C within 36 h. The Yano model is suitable for the prediction of the growth kinetics of strains over the entire PX concentration range. Gas chromatography/mass spectrometry analysis indicated that PX was converted into four and four intermediates in the presence of the strains HN01 and HN02, respectively, and the possible mechanisms were proposed. The results can be used in purifying industrial hydrophobic gaseous VOCs and improving the bioavailability of VOCs with self-produced biosurfactants" |
| Keywords: | "Agar Biodegradation, Environmental Biological Availability *Enterobacter cloacae/metabolism Gases Klebsiella pneumoniae/metabolism Sewage Solubility Surface-Active Agents/chemistry *Volatile Organic Compounds Xylenes Biodegradation Biosurfactants Hydropho;" |
| Notes: | "MedlineWang, Yan Wan, Shungang Yu, Weili Yuan, Dan Sun, Lei eng England 2022/06/15 Chemosphere. 2022 Oct; 304:135328. doi: 10.1016/j.chemosphere.2022.135328. Epub 2022 Jun 11" |
