| Title: | Determination of carnitine and acylcarnitines in urine by high-performance liquid chromatography-electrospray ionization ion trap tandem mass spectrometry |
| Author(s): | Vernez L; Hopfgartner G; Wenk M; Krahenbuhl S; |
| Address: | "Division of Clinical Pharmacology and Toxicology, University Hospital of Basel, Petersgraben 4, 4031 Basel, Switzerland" |
| DOI: | 10.1016/s0021-9673(02)01838-1 |
| ISSN/ISBN: | 0021-9673 (Print) 0021-9673 (Linking) |
| Abstract: | "A high-performance liquid chromatography-mass spectrometry method has been developed for the simultaneous determination of native carnitine and eight acylcarnitines in urine. The procedure uses a solid-phase extraction on a cation-exchange column and the separation is performed without derivatization within 17 min on a reversed-phase C8 column in the presence of a volatile ion-pairing reagent. The detector was an ion trap mass spectrometer and quantification was carried out in the MS-MS mode. Validation was done for aqueous standards at ranges between 0.75 and 200 micromol/l, depending on the compound. Carnitine was quantified in urine and comparison with a radioenzymatic assay gave a satisfactory correlation (R2 = 0.981). The assay could be successfully applied to the diagnostic of pathological acylcarnitines profile of metabolic disorders in urines of patients suffering from different organic acidurias" |
| Keywords: | "Carnitine/*analogs & derivatives/*urine Chromatography, High Pressure Liquid/*methods Reproducibility of Results Spectrometry, Mass, Electrospray Ionization/*methods;" |
| Notes: | "MedlineVernez, Laurence Hopfgartner, Gerard Wenk, Markus Krahenbuhl, Stephan eng Research Support, Non-U.S. Gov't Netherlands 2003/02/05 J Chromatogr A. 2003 Jan 17; 984(2):203-13. doi: 10.1016/s0021-9673(02)01838-1" |
