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J Antibiot (Tokyo)

Title:		Discovering potential Streptomyces hormone producers by using disruptants of essential biosynthetic genes as indicator strains
Author(s):		Thao NB; Kitani S; Nitta H; Tomioka T; Nihira T;
Address:		"International Center for Biotechnology, Osaka University, Osaka, Japan. Faculty of Science, MU-OU Collaborative Research Center for Bioscience and Biotechnology, Mahidol University, Bangkok, Thailand"
Journal Title:		J Antibiot (Tokyo)
Year:		2017
Volume:		20170719
Issue:		10
Page Number:		1004 - 1008
DOI:		10.1038/ja.2017.85
ISSN/ISBN:		1881-1469 (Electronic) 0021-8820 (Linking)
Abstract:		"Autoregulators are low-molecular-weight signaling compounds that control the production of many secondary metabolites in actinomycetes and have been referred to as 'Streptomyces hormones'. Here, potential producers of Streptomyces hormones were investigated in 40 Streptomyces and 11 endophytic actinomycetes. Production of gamma-butyrolactone-type (IM-2, VB) and butenolide-type (avenolide) Streptomyces hormones was screened using Streptomyces lavendulae FRI-5 (DeltafarX), Streptomyces virginiae (DeltabarX) and Streptomyces avermitilis (Deltaaco), respectively. In these strains, essential biosynthetic genes for Streptomyces hormones were disrupted, enabling them to respond solely to the externally added hormones. The results showed that 20% of each of the investigated strains produced IM-2 and VB, confirming that gamma-butyrolactone-type Streptomyces hormones are the most common in actinomycetes. Unlike the gamma-butyrolactone type, butenolide-type Streptomyces hormones have been discovered in recent years, but their distribution has been unclear. Our finding that 24% of actinomycetes (12 of 51 strains) showed avenolide activity revealed for the first time that the butenolide-type Streptomyces hormone is also common in actinomycetes"
Keywords:		*Gene Knockout Techniques Mass Screening Metabolic Networks and Pathways/*genetics Microbiological Techniques Pheromones/chemistry/classification/*metabolism Streptomyces/*metabolism;
Notes:		"MedlineThao, Nguyen B Kitani, Shigeru Nitta, Hiroko Tomioka, Toshiya Nihira, Takuya eng England 2017/09/28 J Antibiot (Tokyo). 2017 Oct; 70(10):1004-1008. doi: 10.1038/ja.2017.85. Epub 2017 Jul 19"