| Title: | "Identification and characterization of pheromone receptors and interplay between receptors and pheromone binding proteins in the diamondback moth, Plutella xyllostella" |
| Author(s): | Sun M; Liu Y; Walker WB; Liu C; Lin K; Gu S; Zhang Y; Zhou J; Wang G; |
| Address: | "State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China" |
| DOI: | 10.1371/journal.pone.0062098 |
| ISSN/ISBN: | 1932-6203 (Electronic) 1932-6203 (Linking) |
| Abstract: | "Moths depend on olfactory cues such as sex pheromones to find and recognize mating partners. Pheromone receptors (PRs) and Pheromone binding proteins (PBPs) are thought to be associated with olfactory signal transduction of pheromonal compounds in peripheral olfactory reception. Here six candidate pheromone receptor genes in the diamondback moth, Plutella xyllostella were identified and cloned. All of the six candidate PR genes display male-biased expression, which is a typical characteristic of pheromone receptors. In the Xenopus-based functional study and in situ hybridization, PxylOR4 is defined as another pheromone receptor in addition to the previously characterized PxylOR1. In the study of interaction between PRs and PBPs, PxylPBPs could increase the sensitivity of the complex expressing oocyte cells to the ligand pheromone component while decreasing the sensitivity to pheromone analogs. We deduce that activating pheromone receptors in olfactory receptor neurons requires some role of PBPs to pheromone/PBP complex. If the chemical signal is not the pheromone component, but instead, a pheromone analog with a similar structure, the complex would have a decreased ability to activate downstream pheromone receptors" |
| Keywords: | Amino Acid Sequence Animals Arthropod Antennae/metabolism Carrier Proteins/*genetics/metabolism Female Gene Expression Insect Proteins/*genetics/metabolism Male Molecular Sequence Data Moths/*genetics/metabolism Olfactory Perception/physiology Oocytes/met; |
| Notes: | "MedlineSun, Mengjing Liu, Yang Walker, William B Liu, Chengcheng Lin, Kejian Gu, Shaohua Zhang, Yongjun Zhou, Jingjiang Wang, Guirong eng Research Support, Non-U.S. Gov't 2013/04/30 PLoS One. 2013 Apr 23; 8(4):e62098. doi: 10.1371/journal.pone.0062098. Print 2013" |
