| Title: | Metabolomic study of volatile compounds emitted by lavender grown under open-field conditions: a potential approach to investigate the yellow decline disease |
| Author(s): | Stierlin E; Nicole F; Costes T; Fernandez X; Michel T; |
| Address: | "Institut de Chimie de Nice, UMR 7272, Universite Cote D'Azur, CNRS, 06108, Nice, France. Laboratoire BVPAM, FRE CNRS INEE, Universite de Lyon, Universite Jean Monnet, 3727 - EA 3061, 23 rue du Dr Paul Michelon, 42000, Saint-Etienne, France. Centre Regionalise Interprofessionnel d'Experimentation en Plantes a Parfum, Aromatiques et Medicinales (CRIEPPAM), Les Quintrands, Route de Volx, 04100, Manosque, France. Institut de Chimie de Nice, UMR 7272, Universite Cote D'Azur, CNRS, 06108, Nice, France. Thomas.MICHEL@univ-cotedazur.fr" |
| DOI: | 10.1007/s11306-020-01654-6 |
| ISSN/ISBN: | 1573-3890 (Electronic) 1573-3882 (Linking) |
| Abstract: | "INTRODUCTION: Fine lavender and lavandin are perfume and medicinal plants originate from the South of France and are widely cultivated for their essential oils. Recently, cultivated plants suffered from a severe decline in France, due to the propagation of the yellow decline disease. This disease is caused by the stolbur phytoplasma, a bacterium transmitted by a sap-sucking insect, the planthopper. OBJECTIVES: In order to understand the complex relationships between host plant, pest, pathogen and environment responsible for the yellow decline of lavender, we use a metabolomic approach to highlight changes in chemical emissions from asymptomatic ('healthy') and symptomatic ('infected') plants. METHODS: Volatile compounds produced by fine lavender and lavandin were collected in the field using a dynamic headspace extraction approach. Afterwards, compounds trapped on Tenax adsorbent were thermodesorbed and analysed using an automated thermal desorption-gas chromatography-mass spectrometry (ATD-GC-MS). Multivariate statistical analyses was performed using principal component analysis and partial least square discriminant analyses. RESULTS: The untargeted screening of volatiles allowed the separation of asymptomatic and symptomatic plants according to their emissions. The approach was sufficiently accurate so as to separate the emissions according to the different stages of infection. Twelve compounds were found to be deregulated metabolites of yellow disease infection, common to fine lavender (variety 7713) and lavandin (variety abrial). CONCLUSION: The metabolomic approach allowed for the effective identification of chemical variations between infected and healthy plants in a complex field environment" |
| Keywords: | Discriminant Analysis Gas Chromatography-Mass Spectrometry Lavandula/*chemistry/growth & development/metabolism *Metabolomics *Plant Diseases Principal Component Analysis Volatile Organic Compounds/analysis/*metabolism Dynamic headspace extraction Gc-ms L; |
| Notes: | "MedlineStierlin, Emilie Nicole, Florence Costes, Thomas Fernandez, Xavier Michel, Thomas eng Research Support, Non-U.S. Gov't 2020/02/28 Metabolomics. 2020 Feb 26; 16(3):31. doi: 10.1007/s11306-020-01654-6" |
