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Bioresour Technol

Title:		Kinetic and molecular analyses reveal isoprene degradation potential of Methylobacterium sp
Author(s):		Srivastva N; Vishwakarma P; Bhardwaj Y; Singh A; Manjunath K; Dubey SK;
Address:		"Department of Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, India. Department of Microbiology and Biotechnology, Bangalore University, Bangalore 560056, India. Department of Botany, Institute of Science, Banaras Hindu University, Varanasi 221005, India. Electronic address: skdubey@bhu.ac.in"
Journal Title:		Bioresour Technol
Year:		2017
Volume:		20170204
Issue:
Page Number:		87 - 91
DOI:		10.1016/j.biortech.2017.02.002
ISSN/ISBN:		1873-2976 (Electronic) 0960-8524 (Linking)
Abstract:		"Efforts were made to isolate and characterize bacteria capable of growing on methane and organic compounds, and to achieve the simultaneous degradation of more than one pollutant. Among the methanotrophs, species of Methylobacterium was able to catabolize a variety of hydrocarbons, including the branched-chain alkenes. Therefore, laboratory incubations experiments were carried out in batch mode to assess the potential of Methylobacterium sp. PV1 for degrading isoprene, the low-molecular-weight alkene, the most abundant non-methane volatile hydrocarbon present in the environment. Methylobacterium sp. PV1, isolated from paddy field soil, was characterized by pmoA and 16S rRNA gene sequencing and FAME analysis, and used for isoprene degradation. The kinetics of biodegradation is studied using the Michaelis-Menten model. The optimum degradation (80%) with maximum average relative degradation rate was observed at 150ppm isoprene. The degradation products were also analyzed using FTIR"
Keywords:		"Biodegradation, Environmental Butadienes/*metabolism Hemiterpenes/*metabolism *Methylobacterium Pentanes/*metabolism Phylogeny RNA, Ribosomal, 16S Biodegradation Ftir Isoprene Kinetics Methylobacterium;"
Notes:		"MedlineSrivastva, Navnita Vishwakarma, P Bhardwaj, Y Singh, A Manjunath, K Dubey, Suresh K eng England 2017/03/04 Bioresour Technol. 2017 Oct; 242:87-91. doi: 10.1016/j.biortech.2017.02.002. Epub 2017 Feb 4"