Title: | Effect of Inoculation with Lentilactobacillus buchneri and Lacticaseibacillus paracasei on the Maize Silage Volatilome: The Advantages of Advanced 2D-Chromatographic Fingerprinting Approaches |
Author(s): | Squara S; Ferrero F; Tabacco E; Cordero C; Borreani G; |
Address: | "Dipartimento di Scienza e Tecnologia del Farmaco, University of Turin, Turin 10124, Italy. Department of Agriculture, Forest and Food Sciences, University of Turin, Grugliasco 10124, TO, Italy" |
ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Print) 0021-8561 (Linking) |
Abstract: | "In this study, the complex volatilome of maize silage samples conserved for 229 d, inoculated with Lentilactobacillus buchneri (Lbuc) and Lacticaseibacillus paracasei (Lpar), is explored by means of advanced fingerprinting methodologies based on comprehensive two-dimensional gas chromatography and time-of-flight mass spectrometry. The combined untargeted and targeted (UT) fingerprinting strategy covers 452 features, 269 of which were putatively identified and assigned within their characteristic classes. The high amounts of short-chain free fatty acids and alcohols were produced by fermentation and led to a large number of esters. The impact of Lbuc fermentation was not clearly distinguishable from the control samples; however, Lpar had a strong and distinctive signature that was dominated by propionic acid and 1-propanol characteristic volatiles. The approach provides a better understanding of silage stabilization mechanisms against the degradative action of yeasts and molds during the exposure of silage to air" |
Keywords: | "1-Propanol Aerobiosis Fatty Acids, Nonesterified Lactobacillus *Lacticaseibacillus paracasei Propionates/analysis *Silage/analysis Zea mays LAB inocula aerobic stability combined untargeted and targeted (UT) fingerprinting comprehensive two-dimensional ga;" |
Notes: | "MedlineSquara, Simone Ferrero, Francesco Tabacco, Ernesto Cordero, Chiara Borreani, Giorgio eng 2022/09/15 J Agric Food Chem. 2022 Sep 28; 70(38):12232-12248. doi: 10.1021/acs.jafc.2c03652. Epub 2022 Sep 14" |