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Int J Mol Sci

Title:		Functional expression of an orchid fragrance gene in Lactococcus lactis
Author(s):		Song AAL; Abdullah JO; Abdullah MP; Shafee N; Rahim RA;
Address:		"Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400, UPM Serdang Selangor, Malaysia. Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400, UPM Serdang, Malaysia. Institute of Bioscience, University Putra Malaysia, 43400, UPM Serdang, Malaysia"
Journal Title:		Int J Mol Sci
Year:		2012
Volume:		20120202
Issue:		2
Page Number:		1582 - 1597
DOI:		10.3390/ijms13021582
ISSN/ISBN:		1422-0067 (Electronic) 1422-0067 (Linking)
Abstract:		"Vanda Mimi Palmer (VMP), an orchid hybrid of Vanda tesselata and Vanda Tan Chay Yan is a highly scented tropical orchid which blooms all year round. Previous studies revealed that VMP produces a variety of isoprenoid volatiles during daylight. Isoprenoids are well known to contribute significantly to the scent of most fragrant plants. They are a large group of secondary metabolites which may possess valuable characteristics such as flavor, fragrance and toxicity and are produced via two pathways, the mevalonate (MVA) pathway or/and the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway. In this study, a sesquiterpene synthase gene denoted VMPSTS, previously isolated from a floral cDNA library of VMP was cloned and expressed in Lactococcus lactis to characterize the functionality of the protein. L. lactis, a food grade bacterium which utilizes the mevalonate pathway for isoprenoid production was found to be a suitable host for the characterization of plant terpene synthases. Through recombinant expression of VMPSTS, it was revealed that VMPSTS produced multiple sesquiterpenes and germacrene D dominates its profile"
Keywords:		"Alkyl and Aryl Transferases/*biosynthesis/genetics *Genes, Plant Lactococcus lactis/*genetics Magnoliopsida/enzymology/*genetics Oils, Volatile/*metabolism Plant Proteins/*biosynthesis/genetics Recombinant Proteins/biosynthesis/genetics Terpenes/metabolis;"
Notes:		"MedlineSong, Adelene Ai Lian Abdullah, Janna O Abdullah, Mohd Puad Shafee, Norazizah Rahim, Raha A eng Research Support, Non-U.S. Gov't Switzerland 2012/03/13 Int J Mol Sci. 2012; 13(2):1582-1597. doi: 10.3390/ijms13021582. Epub 2012 Feb 2"