| Title: | The MADS-box family of transcription factors |
| Address: | "Department of Biochemistry and Genetics, Medical School, University of Newcastle upon Tyne, England" |
| DOI: | 10.1111/j.1432-1033.1995.tb20430.x |
| ISSN/ISBN: | 0014-2956 (Print) 0014-2956 (Linking) |
| Abstract: | "The MADS-box family of transcription factors has been defined on the basis of primary sequence similarity amongst numerous proteins from a diverse range of eukaryotic organisms including yeasts, plants, insects, amphibians and mammals. The MADS-box is a conserved motif found within the DNA-binding domains of these proteins and the name refers to four of the originally identified members: MCM1, AG, DEFA and SRF. Several proteins within this family have significant biological roles. For example, the human serum-response factor (SRF) is involved in co-ordinating transcription of the protooncogene c-fos, whilst MCM1 is central to the transcriptional control of cell-type specific genes and the pheromone response in the yeast Saccharomyces cerevisiae. The RSRF/MEF2 proteins comprise a sub-family of this class of transcription factors which are key components in muscle-specific gene regulation. Moreover, in plants, MADS-box proteins such as AG, DEFA and GLO play fundamental roles during flower development. The MADS-box is a contiguous conserved sequence of 56 amino acids, of which 9 are identical in all family members described so far. Several members have been shown to form dimers and consequently two functional regions within the MADS-box have been defined. The N-terminal half is the major determinant of DNA-binding specificity whilst the C-terminal half is necessary for dimerisation. This organisation allows the potential formation of numerous proteins, with subtly different DNA-binding specificities, from a limited number of genes by heterodimerisation between different MADS-box proteins. The majority of MADS-box proteins bind similar sites based on the consensus sequence CC(A/T)6GG although each protein apparently possesses a distinct binding specificity. Moreover, several MADS-box proteins specifically recruit other transcription factors into multi-component regulatory complexes. Such interactions with other proteins appears to be a common theme within this family and play a pivotal role in the regulation of target genes" |
| Keywords: | "Amino Acid Sequence Animals Conserved Sequence DNA-Binding Proteins/genetics Gene Expression Regulation Molecular Sequence Data Saccharomyces cerevisiae/genetics Sequence Alignment Transcription Factors/*genetics Transcription, Genetic/genetics;" |
| Notes: | "MedlineShore, P Sharrocks, A D eng Wellcome Trust/United Kingdom Research Support, Non-U.S. Gov't Review England 1995/04/01 Eur J Biochem. 1995 Apr 1; 229(1):1-13. doi: 10.1111/j.1432-1033.1995.tb20430.x" |
