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J Exp Bot

Title:		"A 13-lipoxygenase, TomloxC, is essential for synthesis of C5 flavour volatiles in tomato"
Author(s):		Shen J; Tieman D; Jones JB; Taylor MG; Schmelz E; Huffaker A; Bies D; Chen K; Klee HJ;
Address:		"Horticultural Sciences, University of Florida, PO Box 110690, Gainesville, FL 32611-0690, USA"
Journal Title:		J Exp Bot
Year:		2014
Volume:		20140122
Issue:		2
Page Number:		419 - 428
DOI:		10.1093/jxb/ert382
ISSN/ISBN:		1460-2431 (Electronic) 0022-0957 (Print) 0022-0957 (Linking)
Abstract:		"C5 volatile compounds, derived from fatty acids, are among the most important contributors to consumer liking of fresh tomatoes. Despite their important roles in flavour, the genes responsible for C5 volatile synthesis have yet to be identified. This work shows that their synthesis is catalysed in part by a 13-lipoxygenase (LOX), TomloxC, the same enzyme responsible for synthesis of C6 volatiles. C5 synthesis is independent of hydroperoxide lyase (HPL); moreover, HPL knockdown significantly increased C5 volatile synthesis. This LOX-dependent, HPL-independent pathway functions in both fruits and leaves. Synthesis of C5 volatiles increases in leaves following mechanical wounding but does not increase in response to infection with Xanthomonas campestris pv. vesicatoria. Large reductions in C5 and C6 volatiles in antisense TomloxC knockdown plants were observed but those reductions did not alter the development of disease symptoms, indicating that these volatiles do not have an important defensive function against this bacterial pathogen"
Keywords:		"Biosynthetic Pathways Cyclopentanes/metabolism Down-Regulation/genetics Fruit/enzymology/genetics Gene Expression Regulation, Plant Linoleic Acid/metabolism Lipoxygenase/genetics/*metabolism Solanum lycopersicum/*enzymology/genetics/microbiology Oxylipins;"
Notes:		"MedlineShen, Jiyuan Tieman, Denise Jones, Jeffrey B Taylor, Mark G Schmelz, Eric Huffaker, Alisa Bies, Dawn Chen, Kunsong Klee, Harry J eng Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. England 2014/01/24 J Exp Bot. 2014 Feb; 65(2):419-28. doi: 10.1093/jxb/ert382. Epub 2014 Jan 22"