| Title: | Attraction of Adults of Cyclocephala lunulata and Cyclocephala barrerai (Coleoptera: Scarabaeoidea: Melolonthidae) towards Bacteria Volatiles Isolated from Their Genital Chambers |
| Author(s): | Sanchez-Cruz A; Robledo N; Rosete-Enriquez M; Romero-Lopez AA; |
| Address: | "Laboratorio de Ecologia Quimica de Insectos, Centro de Desarrollo de Productos Bioticos, Instituto Politecnico Nacional. Carretera Yautepec-Jojutla, Km. 6, calle CEPROBI No. 8, Col. San Isidro, Yautepec, Morelos C.P. 62731, Mexico. Laboratorio de Macromoleculas, Facultad de Ciencias Biologicas, Benemerita Universidad Autonoma de Puebla, Boulevard Capitan Carlos Camacho Espiritu, Edificio 112-A, Ciudad Universitaria, Col. Jardines de San Manuel, Puebla C. P. 72570, Mexico. Laboratorio de Infoquimicos y Otros compuestos Bioticos, Facultad de Ciencias Biologicas, Benemerita Universidad Autonoma de Puebla, Boulevard Capitan Carlos Camacho Espiritu, Edificio 112-A, Ciudad Universitaria, Col. Jardines de San Manuel, Puebla C. P. 72570, Mexico" |
| DOI: | 10.3390/molecules25194430 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "In the study of the chemical communication of adults of the Melolonthidae family, bacteria have been observed in the epithelium of the genital chamber; possibly, bacteria are involved in the production of sex attractants in their hosts. Therefore, it is important to identify the volatile organic compounds from bacteria (VOCsB) released by these microorganisms and study the biological activity stimulated by VOBCs in adults of Melolonthidae. In this study, bacteria were isolated from the genital chamber of Cyclocephala lunulata and Cyclocephala barrerai, from which VOCsB were extracted using static headspace solid-phase microextraction (SHS-SPME) and dynamic headspace Super Q solid-phase extraction (DHS-SPE) and analyzed using gas chromatography-mass spectrometry. The effect of VOCsB on the hosts and conspecifics was evaluated utilizing an olfactometer and electroantennography (EAG). Two species of Enterobacteria were isolated from the genital chamber of each female species, and VOCsB derived from sulfur-containing compounds, alcohols, esters, and fatty acids were identified. An attraction response was observed in olfactometry studies, and antennal responses to VOCsB were confirmed in EAG bioassays. With these results, new perspectives on the relationship between these beetles and their bacteria emerge, in addition to establishing a basis for management programs in the future" |
| Keywords: | Animals Bacteria/*chemistry/growth & development/isolation & purification Biological Assay Coleoptera/*microbiology/*physiology Female Genitalia/*microbiology Male *Sex Attractants/chemistry/isolation & purification/pharmacology Solid Phase Microextractio; |
| Notes: | "MedlineSanchez-Cruz, Abraham Robledo, Norma Rosete-Enriquez, Maria Romero-Lopez, Angel A eng Switzerland 2020/10/01 Molecules. 2020 Sep 27; 25(19):4430. doi: 10.3390/molecules25194430" |
