| Title: | Early and late trisporoids differentially regulate beta-carotene production and gene transcript Levels in the mucoralean fungi Blakeslea trispora and Mucor mucedo |
| Author(s): | Sahadevan Y; Richter-Fecken M; Kaerger K; Voigt K; Boland W; |
| Address: | "Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Jena, Germany" |
| ISSN/ISBN: | 1098-5336 (Electronic) 0099-2240 (Print) 0099-2240 (Linking) |
| Abstract: | "The multistep cleavage of carotenoids in Mucorales during the sexual phase results in a cocktail of trisporic acid (C18) sex pheromones. We hypothesized that the C18 trisporoid intermediates have a specific regulatory function for sex pheromone production and carotenogenesis that varies with genus/species and vegetative and sexual phases of their life cycles. Real-time quantitative PCR kinetics determined for Blakeslea trispora displayed a very high transcript turnover in the gene for carotenoid cleavage dioxygenase, tsp3, during the sexual phase. An in vivo enzyme assay and chromatographic analysis led to the identification of beta-apo-12'-carotenal as the first apocarotenoid involved in trisporic acid biosynthesis in B. trispora. Supplementation of C18 trisporoids, namely D'orenone, methyl trisporate C, and trisporin C, increased tsp3 transcripts in the plus compared to minus partners. Interestingly, the tsp1 gene, which is involved in trisporic acid biosynthesis, was downregulated compared to tsp3 irrespective of asexual or sexual phase. Only the minus partners of both B. trispora and Mucor mucedo had enhanced beta-carotene production after treatment with C20 apocarotenoids, 15 different trisporoids, and their analogues. We conclude that the apocarotenoids and trisporoids influence gene transcription and metabolite production, depending upon the fungal strain, corresponding genus, and developmental phase, representing a 'chemical dialect' during sexual communication" |
| Keywords: | "Biosynthetic Pathways/*drug effects Fatty Acids, Unsaturated/*metabolism Gene Expression Profiling Gene Expression Regulation, Fungal/*drug effects Mucorales/*drug effects Real-Time Polymerase Chain Reaction beta Carotene/*metabolism;" |
| Notes: | "MedlineSahadevan, Yamuna Richter-Fecken, Mareike Kaerger, Kerstin Voigt, Kerstin Boland, Wilhelm eng Research Support, Non-U.S. Gov't 2013/09/24 Appl Environ Microbiol. 2013 Dec; 79(23):7466-75. doi: 10.1128/AEM.02096-13. Epub 2013 Sep 20" |
