| Title: | The effects of maternal diabetes and insulin treatment on neurogenesis in the developing hippocampus of male rats |
| Author(s): | Sadeghi A; Esfandiary E; Hami J; Khanahmad H; Hejazi Z; Mardani M; Razavi S; |
| Address: | "Cellular and molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran; Department of Anatomical Sciences, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran. Electronic address: akramsadeghi944@gmail.com. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Cellular and molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran; Department of Anatomical Sciences, School of Medicine, Birjand University of Medical Sciences, Birjand, Iran. Department of Genetic and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran. Electronic address: razav@medmui.ac.ir" |
| DOI: | 10.1016/j.jchemneu.2018.03.005 |
| ISSN/ISBN: | 1873-6300 (Electronic) 0891-0618 (Linking) |
| Abstract: | "Diabetes in pregnancy is associated with an increasing risk of congenital malformations and central nervous system disorders (CNS) especially hippocampal neuronal circuitry disruption as a discreet region involved in neurogenesis phenomenon. This study aimed to investigate the effect of maternal diabetes and insulin treatment on the expression and distribution pattern of NeuN and DCX as two important markers of neurogenesis paradigm in developing rat hippocampus. All animals were randomly divided into three groups as follows: Control group, Diabetic (STZ-D), Diabetic treated with insulin (STZ-INS). Diabetes was induced in Wistar female rats by Sterptozotocin intraperitoneal injection (single does). Following confirmation of diabetes, animals were mated with non-diabetic males. Four to six units of protamine-Zinc insulin were delivered subcutaneously (SC) in insulin treated group. At the post-natal day 14 (P14), the brain of male offspring's were removed for further study. In fact Immunofluorescence staining and Real time - PCR assays are used for evaluation of neurogenesis phenomenon. Our results showed a significant higher level of hippocampal DCX expression and an increase in the mean number of DCX positive cells in the DG of diabetic group male offspring (P?ª+?ª+0.05). Nevertheless the results of immunofluorescence staining for NeuN also indicated that the mean number of NeuN+ cells was significantly lower in dentate gyrus of diabetic group male offspring (P?ª+ |
| Keywords: | "Animals Diabetes Mellitus, Experimental/*complications Doublecortin Protein Female *Hippocampus/drug effects Hypoglycemic Agents/*pharmacology Insulin/*pharmacology Male Neurogenesis/drug effects Pregnancy Pregnancy Complications/*drug therapy Rats Rats, ;" |
| Notes: | "MedlineSadeghi, Akram Esfandiary, Ebrahim Hami, Javad Khanahmad, Hossein Hejazi, Zahra Mardani, Mohammad Razavi, Shahnaz eng Research Support, Non-U.S. Gov't Netherlands 2018/03/27 J Chem Neuroanat. 2018 Sep; 91:27-34. doi: 10.1016/j.jchemneu.2018.03.005. Epub 2018 Mar 22" |
