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Curr Protoc

Title:		Measurement of the Effects of Metals on Taxis-to-Food Behavior in Caenorhabditis elegans
Author(s):		Queiros L; Monteiro L; Marques C; Pereira JL; Goncalves FJM; Aschner M; Pereira P;
Address:		"Department of Biology & CESAM (Centre for Environmental and Marine Studies), University of Aveiro, Aveiro, Portugal. Marine Biology Research Group, Biology Department, Faculty of Sciences, Ghent University, Ghent, Belgium. Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, New York"
Journal Title:		Curr Protoc
Year:		2021
Volume:		1
Issue:		5
Page Number:		e131 -
DOI:		10.1002/cpz1.131
ISSN/ISBN:		2691-1299 (Electronic) 2691-1299 (Linking)
Abstract:		"Chemosensation in nematodes is linked to processes that affect their ability to survive, such as the search for food and the avoidance of toxic substances. Since the 1970s, numerous studies have assessed chemotaxis in the nematode species Caenorhabditis elegans, focusing on a multitude of agents, including bacteria (food), ions, salts, hormones, volatile organic compounds, and, to a lesser extent, metal-contaminated medium/food. The few studies evaluating metal exposure have reported a variety of responses (neutral, attraction, avoidance), which generally appear to be contaminant and/or concentration specific. Differences in experimental designs, however, hinder appropriate comparison of the findings and attainment of firm conclusions. Therefore, we herein propose and describe a detailed protocol for the assessment of the effects of metals on taxis-to-food behavior in C. elegans. Distinct approaches are proposed in two innovative stages of testing to (1) screen metals' effects on taxis-to-food behavior and (2) classify the behavioral response as attraction/avoidance/indifference or preference. Use of such a standard protocol will allow for easy comparison across studies and direct interpretation of results. Findings using this model system can contribute to a deeper understanding of the real risks of metal contamination to nematodes and how such contaminants could impact ecosystems in general, given the key environmental roles that these organisms play. (c) 2021 Wiley Periodicals LLC. Basic Protocol: Assessing the effects of metal contamination on taxis-to-food behavior in Caenorhabditis elegans Support Protocol 1: Synchronization of C. elegans by hand-picking gravid worms Support Protocol 2: Synchronization of C. elegans by using a bleaching solution"
Keywords:		Animals Caenorhabditis elegans *Caenorhabditis elegans Proteins Chemotaxis Ecosystem *Nematoda attraction avoidance metals preference;
Notes:		"MedlineQueiros, Libania Monteiro, Luana Marques, Carlos Pereira, Joana L Goncalves, Fernando J M Aschner, Michael Pereira, Patricia eng R01 ES007331/ES/NIEHS NIH HHS/ R01 ES010563/ES/NIEHS NIH HHS/ 2021/05/12 Curr Protoc. 2021 May; 1(5):e131. doi: 10.1002/cpz1.131"