| Title: | "Intercellular signaling in Stigmatella aurantiaca: purification and characterization of stigmolone, a myxobacterial pheromone" |
| Author(s): | Plaga W; Stamm I; Schairer HU; |
| Address: | "Zentrum fur Molekulare Biologie der Universitat Heidelberg (ZMBH), Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany. wplaga@sun0.urz.uni-heidelberg.de" |
| ISSN/ISBN: | 0027-8424 (Print) 1091-6490 (Electronic) 0027-8424 (Linking) |
| Abstract: | "The myxobacterium Stigmatella aurantiaca passes through a life cycle that involves formation of a multicellular fruiting body as the most complex stage. An early step in this differentiation process depends on a signal factor secreted by the cells when nutrients become limited. The formation of a fruiting body from a small cell population can be accelerated by addition of this secreted material. The bioactive compound was found to be steam volatile. It was purified to homogeneity by steam distillation followed by reversed-phase and normal-phase HPLC. The pheromone was named stigmolone, in accordance with the structure 2,5, 8-trimethyl-8-hydroxy-nonan-4-one, as determined by NMR and mass spectrometry. Stigmolone represents a structurally unique and highly bioactive prokaryotic pheromone that is effective in the bioassay at 1 nM concentration" |
| Keywords: | "Alkanes/chemistry Cell Aggregation/drug effects/physiology Cell Differentiation/physiology Chromatography, High Pressure Liquid Ketones/chemistry Magnetic Resonance Spectroscopy Mass Spectrometry Molecular Structure Myxococcales/chemistry/*physiology Pher;" |
| Notes: | "MedlinePlaga, W Stamm, I Schairer, H U eng 1998/09/16 Proc Natl Acad Sci U S A. 1998 Sep 15; 95(19):11263-7. doi: 10.1073/pnas.95.19.11263" |
