| Title: | Breath Collection from Children for Disease Biomarker Discovery |
| Author(s): | Berna AZ; DeBosch B; Stoll J; Odom John AR; |
| Address: | "Department of Pediatrics, Washington University School of Medicine. Department of Pediatrics, Washington University School of Medicine; Cell Biology & Physiology, Washington University School of Medicine. Division of Gastroenterology, Hepatology and Nutrition, Washington University School of Medicine. Department of Pediatrics, Washington University School of Medicine; Department of Molecular Microbiology, Washington University School of Medicine; aodom@wustl.edu" |
| ISSN/ISBN: | 1940-087X (Electronic) 1940-087X (Linking) |
| Abstract: | "Breath collection and analysis can be used to discover volatile biomarkers in a number of infectious and non-infectious diseases, such as malaria, tuberculosis, lung cancer, and liver disease. This protocol describes a reproducible method for sampling breath in children and then stabilizing breath samples for further analysis with gas chromatography-mass spectrometry (GC-MS). The goal of this method is to establish a standardized protocol for the acquisition of breath samples for further chemical analysis, from children aged 4-15 years. First, breath is sampled using a cardboard mouthpiece attached to a 2-way valve, which is connected to a 3 L bag. Breath analytes are then transferred to a thermal desorption tube and stored at 4-5 degrees C until analysis. This technique has been previously used to capture breath of children with malaria for successful breath biomarker identification. Subsequently, we have successfully applied this technique to additional pediatric cohorts. The advantage of this method is that it requires minimal cooperation on part of the patient (of particular value in pediatric populations), has a short collection period, does not require trained staff, and can be performed with portable equipment in resource-limited field settings" |
| Keywords: | "Adolescent Biomarkers/analysis Breath Tests/instrumentation/*methods Child Child, Preschool Gas Chromatography-Mass Spectrometry Humans Malaria/diagnosis Volatile Organic Compounds/*analysis;" |
| Notes: | "MedlineBerna, Amalia Z DeBosch, Brian Stoll, Janis Odom John, Audrey R eng R01 AI103280/AI/NIAID NIH HHS/ R21 AI123808/AI/NIAID NIH HHS/ R21 AI144472/AI/NIAID NIH HHS/ Research Support, Non-U.S. Gov't Video-Audio Media 2019/03/05 J Vis Exp. 2019 Feb 14; (144):10.3791/59217. doi: 10.3791/59217" |
