| Title: | "Integrated PTR-ToF-MS, GWAS and biological pathway analyses reveal the contribution of cow's genome to cheese volatilome" |
| Author(s): | Pegolo S; Bergamaschi M; Gasperi F; Biasioli F; Cecchinato A; Bittante G; |
| Address: | "Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padua, Viale dell'Universita 16, 35020, Legnaro, Padua, Italy. sara.pegolo@unipd.it. Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padua, Viale dell'Universita 16, 35020, Legnaro, Padua, Italy. Department of Food Quality and Nutrition, Research and Innovation Centre, Fondazione Edmund Mach (FEM), Via E. Mach 1, 38010, San Michele all'Adige, TN, Italy" |
| DOI: | 10.1038/s41598-018-35323-5 |
| ISSN/ISBN: | 2045-2322 (Electronic) 2045-2322 (Linking) |
| Abstract: | "Volatile organic compounds (VOCs) are small molecules that contribute to the distinctive flavour of cheese which is an important attribute for consumer acceptability. To investigate whether cow's genetic background might contribute to cheese volatilome, we carried out genome-wide association studies (GWAS) and pathway-based analyses for 173 spectrometric peaks tentatively associated with several VOCs obtained from proton-transfer-reaction mass spectrometry (PTR-ToF-MS) analyses of 1,075 model cheeses produced using raw whole-milk from Brown Swiss cows. Overall, we detected 186 SNPs associated with 120 traits, several of which mapped close to genes involved in protein (e.g. CSN3, GNRHR and FAM169A), fat (e.g. AGPAT3, SCD5, and GPAM) and carbohydrate (e.g. B3GNT2, B4GALT1, and PHKB) metabolism. Gene set enrichment analysis showed that pathways connected with proteolysis/amino acid metabolism (purine and nitrogen metabolism) as well as fat metabolism (long-term potentiation) and mammary gland function (tight junction) were overrepresented. Our results provide the first evidence of a putative link between cow's genes and cheese flavour and offer new insights into the role of potential candidate loci and the biological functions contributing to the cheese volatilome" |
| Keywords: | "Animals Cattle Cheese/*analysis Female Genome *Genome-Wide Association Study Genotype Mass Spectrometry/*methods Milk/*chemistry Milk Proteins/*genetics/metabolism Phenotype *Polymorphism, Single Nucleotide Protons Quantitative Trait Loci Volatile Organic;" |
| Notes: | "MedlinePegolo, Sara Bergamaschi, Matteo Gasperi, Flavia Biasioli, Franco Cecchinato, Alessio Bittante, Giovanni eng Research Support, Non-U.S. Gov't England 2018/11/20 Sci Rep. 2018 Nov 19; 8(1):17002. doi: 10.1038/s41598-018-35323-5" |
