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J Biol Chem

Title:		Cell-cell recognition in yeast. Characterization of the sexual agglutination factors from Saccharomyces kluyveri
Author(s):		Pierce M; Ballou CE;
Address:
Journal Title:		J Biol Chem
Year:		1983
Volume:		258
Issue:		6
Page Number:		3576 - 3582
DOI:
ISSN/ISBN:		0021-9258 (Print) 0021-9258 (Linking)
Abstract:		"The cell surface molecules responsible for sexual agglutination between haploid cells of opposite mating type from Saccharomyces kluyveri have been purified and characterized. The 17-factor, released from 17-cells by beta-glucanase digestion (Zymolyase), is a glycoprotein of 6 X 10(4) Da. Its binding activity is heat- and protease-labile, but it is stable to reducing agents and exo-alpha-mannosidase digestion. The 16-factor, released from 16-cells by Zymolyase digestion, has a molecular weight of 5 X 10(5) and is over 95% carbohydrate. An active binding fragment can be released from 16-factor, from the factor purified from a mutant of 16-cells (16(mnn1)-factor), and from the surfaces of the cells themselves by dithiothreitol treatment. The 16(mnn1)-binding fragment has a molecular weight of 2 X 10(4) and is 30% carbohydrate. Its binding activity is stable to heat and some proteases, but it is labile to pronase, carboxypeptidases A and Y, alpha-mannosidases, and mild periodate treatment. 125I-16(mnn1)-binding fragment adheres specifically to 17-cells but does not bind to 16-cells or cells of other yeast strains. The binding of the labeled fragment to 17-cells is characterized by a KA of 10(8) M-1, and 5 X 10(5) binding sites are present per cell. The purified intact factors are monovalent and appear to interact in a lock and key fashion to cause the specific agglutination of S. kluyveri 16- and 17-cells"
Keywords:		Amino Acids/analysis *Cell Communication Haploidy Kinetics Mating Factor Molecular Weight Peptide Hydrolases Peptides/*isolation & purification Saccharomyces/*physiology Species Specificity;
Notes:		"MedlinePierce, M Ballou, C E eng 5 F32 GM07369/GM/NIGMS NIH HHS/ AI-12522/AI/NIAID NIH HHS/ Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. 1983/03/25 J Biol Chem. 1983 Mar 25; 258(6):3576-82"