| Title: | Cdc1 is required for growth and Mn2+ regulation in Saccharomyces cerevisiae |
| Address: | "Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710, USA" |
| DOI: | 10.1093/genetics/148.4.1777 |
| ISSN/ISBN: | 0016-6731 (Print) 0016-6731 (Linking) |
| Abstract: | "Cdc1 function was initially implicated in bud formation and nuclear division because cdc1(Ts) cells arrested with a small bud, duplicated DNA, and undivided nucleus. Our studies show that Cdc1 is necessary for cell growth at several stages of the cell cycle, as well as in pheromone-treated cells. Thus, Cdc1 depletion might affect bud formation and nuclear division, as well as other cellular processes, by blocking a process involved in general cell growth. Cells depleted of intracellular Mn2+ also exhibit a cdc1-like phenotype and recent results suggested Cdc1 might be a Mn2+-dependent protein. We show that all of the conditional Cdc1(Ts) alleles tested cause cells to become sensitive to Mn2+ depletion. In addition, Cdc1 overproduction alleviates the chelator sensitivity of several Mn2+ homeostasis mutants. These findings are compatible with a model in which Cdc1 regulates intracellular, and in particular cytosolic, Mn2+ levels which, in turn, are necessary for cell growth" |
| Keywords: | "Carrier Proteins/genetics *Cation Transport Proteins Cell Cycle Proteins/genetics/*physiology Cell Division Cell Wall/physiology DNA, Fungal/metabolism Egtazic Acid/pharmacology Glycosylation Golgi Apparatus Intracellular Fluid Manganese/*metabolism Membr;" |
| Notes: | "MedlinePaidhungat, M Garrett, S eng 1998/04/30 Genetics. 1998 Apr; 148(4):1777-86. doi: 10.1093/genetics/148.4.1777" |
