| Title: | Detection and Imaging of the Plant Pathogen Response by Near-Infrared Fluorescent Polyphenol Sensors |
| Author(s): | Nissler R; Muller AT; Dohrman F; Kurth L; Li H; Cosio EG; Flavel BS; Giraldo JP; Mithofer A; Kruss S; |
| Address: | "Physical Chemistry II, Bochum University, Universitatsstrasse 150, 44801, Bochum, Germany. Institute of Physical Chemistry, Georg-August Universitat Gottingen, Tammannstrasse 6, 37077, Gottingen, Germany. Research Group Plant Defense Physiology, Max Planck Institute for Chemical Ecology, Hans-Knoll-Strasse 8, 07745, Jena, Germany. Institute of Nanotechnology, Karlsruhe Institute of Technology (KIT), 76344, Eggenstein-Leopoldshafen, Germany. Institute for Nature Earth and Energy (INTE-PUCP), Pontifical Catholic University of Peru, Av. Universitaria 1801, San Miguel, 15088, Lima, Peru. Department of Botany and Plant Sciences, University of California, Riverside, CA, 92507, USA. Fraunhofer Institute for Microelectronic Circuits and Systems, Finkenstrasse 61, 47057, Duisburg, Germany" |
| ISSN/ISBN: | 1521-3773 (Electronic) 1433-7851 (Print) 1433-7851 (Linking) |
| Abstract: | "Plants use secondary metabolites such as polyphenols for chemical defense against pathogens and herbivores. Despite their importance in plant pathogen interactions and tolerance to diseases, it remains challenging to detect polyphenols in complex plant tissues. Here, we create molecular sensors for plant polyphenol imaging that are based on near-infrared (NIR) fluorescent single-wall carbon nanotubes (SWCNTs). We identified polyethylene glycol-phospholipids that render (6,5)-SWCNTs sensitive (K(d) =90 nM) to plant polyphenols (tannins, flavonoids, ...), which red-shift (up to 20 nm) and quench their emission (ca. 1000 nm). These sensors report changes in total polyphenol level after herbivore or pathogen challenge in crop plant systems (Soybean Glycine max) and leaf tissue extracts (Tococa spp.). We furthermore demonstrate remote chemical imaging of pathogen-induced polyphenol release from roots of soybean seedlings over the time course of 24 h. This approach allows in situ visualization and understanding of the chemical plant defense in real time and paves the way for plant phenotyping for optimized polyphenol secretion" |
| Keywords: | *Polyphenols biosensors carbon nanotubes imaging near-infrared fluorescence plant polyphenols; |
| Notes: | "MedlineNissler, Robert Muller, Andrea T Dohrman, Frederike Kurth, Larissa Li, Han Cosio, Eric G Flavel, Benjamin S Giraldo, Juan Pablo Mithofer, Axel Kruss, Sebastian eng volkswagen foundation/ deutsche forschungsgemeinschaft/ studienstiftung des deutschen volkes/ deutscher akademischer austauschdienst/ Research Support, Non-U.S. Gov't Germany 2021/10/06 Angew Chem Int Ed Engl. 2022 Jan 10; 61(2):e202108373. doi: 10.1002/anie.202108373. Epub 2021 Nov 22" |
