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J Chromatogr B Analyt Technol Biomed Life Sci

Title:		In vitro profiling of volatile organic compounds released by Simpson-Golabi-Behmel syndrome adipocytes
Author(s):		Mochalski P; Diem E; Unterkofler K; Mundlein A; Drexel H; Mayhew CA; Leiherer A;
Address:		"Institute for Breath Research, University of Innsbruck, Rathausplatz 4, A-6850 Dornbirn, Austria; Institute of Chemistry, Jan Kochanowski University, Swietokrzyska 15G, PL-25406 Kielce, Poland. Electronic address: pawel.mochalski@uibk.ac.at. MCI Management Center Innsbruck, Universitaetsstrasse 15, A-6020 Innsbruck, Austria. Institute for Breath Research, University of Innsbruck, Rathausplatz 4, A-6850 Dornbirn, Austria; Vorarlberg University of Applied Sciences, Hochschulstrasse 1, A-6850 Dornbirn, Austria. Vorarlberg Institute for Vascular Investigation and Treatment (VIVIT), Carinagasse 47, A-6800 Feldkirch, Austria. Vorarlberg Institute for Vascular Investigation and Treatment (VIVIT), Carinagasse 47, A-6800 Feldkirch, Austria; Division of Angiology, Swiss Cardiovascular Center, University Hospital Berne, Berne, Switzerland; Drexel University College of Medicine, Philadelphia, PA, USA. Institute for Breath Research, University of Innsbruck, Rathausplatz 4, A-6850 Dornbirn, Austria; Molecular Physics Group, School of Physics and Astronomy, University of Birmingham, Edgbaston, Birmingham, B15 2TT, UK. Vorarlberg Institute for Vascular Investigation and Treatment (VIVIT), Carinagasse 47, A-6800 Feldkirch, Austria; Private University of the Principality of Liechtenstein, Triesen, Liechtenstein; Medical Central Laboratories, A-6800 Feldkirch, Austria"
Journal Title:		J Chromatogr B Analyt Technol Biomed Life Sci
Year:		2019
Volume:		20181128
Issue:
Page Number:		256 - 261
DOI:		10.1016/j.jchromb.2018.11.028
ISSN/ISBN:		1873-376X (Electronic) 1570-0232 (Linking)
Abstract:		"Breath analysis offers a non-invasive and rapid diagnostic method for detecting various volatile organic compounds that could be indicators for different diseases, particularly metabolic disorders including type 2 diabetes mellitus. The development of type 2 diabetes mellitus is closely linked to metabolic dysfunction of adipose tissue and adipocytes. However, the VOC profile of human adipocytes has not yet been investigated. Gas chromatography with mass spectrometric detection and head-space needle trap extraction (two-bed Carbopack X/Carboxen 1000 needle traps) were applied to profile VOCs produced and metabolised by human Simpson Golabi Behmel Syndrome adipocytes. In total, sixteen compounds were identified to be related to the metabolism of the cells. Four sulphur compounds (carbon disulphide, dimethyl sulphide, ethyl methyl sulphide and dimethyl disulphide), three heterocyclic compounds (2-ethylfuran, 2-methyl-5-(methyl-thio)-furan, and 2-pentylfuran), two ketones (acetone and 2-pentanone), two hydrocarbons (isoprene and n-heptane) and one ester (ethyl acetate) were produced, and four aldehydes (2-methyl-propanal, butanal, pentanal and hexanal) were found to be consumed by the cells of interest. This study presents the first profile of VOCs formed by human adipocytes, which may reflect the activity of the adipose tissue enzymes and provide evidence of their active role in metabolic regulation. Our data also suggest that a previously reported increase of isoprene and sulphur compounds in diabetic patients may be explained by their production by adipocytes. Moreover, the unique features of this profile, including a high emission of dimethyl sulphide and the production of furan-containing VOCs, increase our knowledge about metabolism in adipose tissue and provide diagnostic potential for future applications"
Keywords:		"Adipocytes/*metabolism Arrhythmias, Cardiac/*metabolism Biomarkers/analysis/metabolism Cells, Cultured Gas Chromatography-Mass Spectrometry Genetic Diseases, X-Linked/*metabolism Gigantism/*metabolism Heart Defects, Congenital/*metabolism Humans Intellect;"
Notes:		"MedlineMochalski, Pawel Diem, Eva Unterkofler, Karl Mundlein, Axel Drexel, Heinz Mayhew, Chris A Leiherer, Andreas eng Netherlands 2018/12/12 J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Jan 1; 1104:256-261. doi: 10.1016/j.jchromb.2018.11.028. Epub 2018 Nov 28"