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J Biol Rhythms

Title:		An antennal circadian clock and circadian rhythms in peripheral pheromone reception in the moth Spodoptera littoralis
Author(s):		Merlin C; Lucas P; Rochat D; Francois MC; Maibeche-Coisne M; Jacquin-Joly E;
Address:		"UMR 1272 INRA-UPMC-AgroParisTech 'Physiologie de l'Insecte: Signalisation et Communication,' INRA Centre de Versailles, Versailles cedex, France"
Journal Title:		J Biol Rhythms
Year:		2007
Volume:		22
Issue:		6
Page Number:		502 - 514
DOI:		10.1177/0748730407307737
ISSN/ISBN:		0748-7304 (Print) 0748-7304 (Linking)
Abstract:		"Circadian rhythms are observed in mating behaviors in moths: females emit sex pheromones and males are attracted by these pheromones in rhythmic fashions. In the moth Spodoptera littoralis, we demonstrated the occurrence of a circadian oscillator in the antenna, the peripheral olfactory organ. We identified different clock genes, period (per), cryptochrome1 (cry1) and cryptochrome2 (cry2), in this organ. Using quantitative real-time PCR (qPCR), we found that their corresponding transcripts cycled circadianly in the antenna as well as in the brain. Electroantennogram (EAG) recordings over 24 h demonstrated for the first time a circadian rhythm in antennal responses of a moth to sex pheromone. qPCR showed that out of one pheromone-binding protein (PBP), one olfactory receptor (OR), and one odorant-degrading enzyme (ODE), all putatively involved in the pheromone reception, only the ODE transcript presented a circadian rhythm that may be related to rhythms in olfactory signal resolution. Peripheral or central circadian clock control of olfaction is then discussed in light of recent data"
Keywords:		Amino Acid Sequence Animals *Biological Clocks Circadian Rhythm/*physiology DNA Primers Darkness Electrochemistry Insect Proteins/chemistry/genetics Insecta/classification/genetics Light Male Mice/classification/genetics Molecular Sequence Data Phylogeny;
Notes:		"MedlineMerlin, Christine Lucas, Philippe Rochat, Didier Francois, Marie-Christine Maibeche-Coisne, Martine Jacquin-Joly, Emmanuelle eng Research Support, Non-U.S. Gov't 2007/12/07 J Biol Rhythms. 2007 Dec; 22(6):502-14. doi: 10.1177/0748730407307737"