| Title: | Cyanamide phytotoxicity in soybean (Glycine max) seedlings involves aldehyde dehydrogenase inhibition and oxidative stress |
| Author(s): | Maninang JS; Okazaki S; Fujii Y; |
| ISSN/ISBN: | 1934-578X (Print) 1555-9475 (Linking) |
| Abstract: | "The phytotoxic effect of the allelochemical cyanamide has been well-documented yet the underlying mechanism for this phenomenon has not been fully characterized. Cognizant of the putative inhibitory effect of cyanamide on aldehyde dehydrogenases (ALDHs), we hereby show that the capacity of mitochondrial preparations from cyanamide-treated soybean seedlings to oxidize acetaldehyde and succinic-semialdehyde was dose-dependently reduced to at most 55% and 70%, respectively. Cyanamide-treated plants exhibited oxidative stress (i.e. increased lipid peroxidation and H2O2 accumulation) that was exacerbated upon exposure to UV-A--symptoms reminiscent of ALDH and succinic-semialdehyde dehydrogenase (SSADH) knock-out Arabidopsis mutants. We suggest that the inhibition of mitochondrial ALDH and SSADH may be a contributory mechanism to the burst in oxidative stress mediated by cyanamide" |
| Keywords: | Aldehyde Dehydrogenase/*antagonists & inhibitors/genetics/metabolism Cyanamide/*toxicity Enzyme Inhibitors/*pharmacology Hydrogen Peroxide/metabolism Lipid Peroxidation/drug effects Mitochondria/drug effects/metabolism Oxidative Stress/*drug effects Phero; |
| Notes: | "MedlineManinang, John S Okazaki, Shin Fujii, Yoshiharu eng 2015/06/11 Nat Prod Commun. 2015 May; 10(5):743-6" |
