| Title: | Analysis of VOCs in Urine Samples Directed towards of Bladder Cancer Detection |
| Author(s): | Ligor T; Adamczyk P; Kowalkowski T; Ratiu IA; Wenda-Piesik A; Buszewski B; |
| Address: | "Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, 87-100 Torun, Poland. Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 87-100 Torun, Poland. Department of General and Oncologic Urology, Nicolaus Copernicus Hospital in Torun, 87-100 Torun, Poland. 'Raluca Ripan' Institute for Research in Chemistry, Babes-Bolyai University, 30 Fantanele, RO-400239 Cluj-Napoca, Romania. Department of Agronomics, Faculty of Agriculture and Biotechnology, Bydgoszcz University of Science and Technology, 85-796 Bydgoszcz, Poland" |
| DOI: | 10.3390/molecules27155023 |
| ISSN/ISBN: | 1420-3049 (Electronic) 1420-3049 (Linking) |
| Abstract: | "Bladder cancer is one of most common types of cancer diagnosed in the genitourinary tract. Typical tests are costly and characterized by low sensitivity, which contributes to a growing interest in volatile biomarkers. Head space solid phase microextraction (SPME) was applied for the extraction of volatile organic compounds from urine samples, and gas chromatography time of flight mass spectrometry (GCxGC TOF MS) was used for the separation and detection of urinary volatiles. A cohort of 40 adult patients with bladder cancer and 57 healthy persons was recruited. Different VOC profiles were obtained for urine samples taken from each group. Twelvecompounds were found only in the samples from theBC group.The proposed candidate biomarkers are butyrolactone; 2-methoxyphenol; 3-methoxy-5-methylphenol; 1-(2,6,6-trimethylcyclohexa-1,3-dien-1-yl)-2-buten-1-one; nootkatone and 1-(2,6,6-trimethyl-1-cyclohexenyl)-2-buten-1-one.Since most of the studies published in the field are proving the potential of VOCs detected in urine samples for the screening and discrimination of patients with bladder cancer from healthy, but rarely presenting the identity of proposed biomarkers, our study represents a novel approach" |
| Keywords: | Adult Biomarkers Gas Chromatography-Mass Spectrometry/methods Humans Rare Diseases Solid Phase Microextraction/methods *Urinary Bladder Neoplasms/diagnosis *Volatile Organic Compounds/analysis GCxGC TOF MS bladder cancer urine analyses volatile organic co; |
| Notes: | "MedlineLigor, Tomasz Adamczyk, Przemyslaw Kowalkowski, Tomasz Ratiu, Ileana Andreea Wenda-Piesik, Anna Buszewski, Boguslaw eng This work was supported by Torun Center of Excellence 'Towards Personalized Medi-cine' operating under Excellence Initiative-Research University/Initiative-Research University/ Switzerland 2022/08/13 Molecules. 2022 Aug 7; 27(15):5023. doi: 10.3390/molecules27155023" |
