| Title: | Gene Identification of Pheromone Gland Genes Involved in Type II Sex Pheromone Biosynthesis and Transportation in Female Tea Pest Ectropis grisescens |
| Author(s): | Li ZQ; Ma L; Yin Q; Cai XM; Luo ZX; Bian L; Xin ZJ; He P; Chen ZM; |
| Address: | "Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, People's Republic of China. Jiangxi Key Laboratory of Bioprocess Engineering and Co-Innovation Center for In-vitro Diagnostic Reagents and Devices, College of Life Sciences, Jiangxi Science & Technology Normal University, Nanchang 330013, People's Republic of China. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing, Jiangsu Province 210014, People's Republic of China. State Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Guiyang, 550025, People's Republic of China zmchen2006@163.com phe1@gzu.edu.cn. Key Laboratory of Tea Biology and Resource Utilization, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, People's Republic of China zmchen2006@163.com phe1@gzu.edu.cn" |
| ISSN/ISBN: | 2160-1836 (Electronic) 2160-1836 (Linking) |
| Abstract: | "Moths can biosynthesize sex pheromones in the female sex pheromone glands (PGs) and can distinguish species-specific sex pheromones using their antennae. However, the biosynthesis and transportation mechanism for Type II sex pheromone components has rarely been documented in moths. In this study, we constructed a massive PG transcriptome database (14.72 Gb) from a moth species, Ectropis grisescens, which uses type II sex pheromones and is a major tea pest in China. We further identified putative sex pheromone biosynthesis and transportation-related unigenes: 111 cytochrome P450 monooxygenases (CYPs), 25 odorant-binding proteins (OBPs), and 20 chemosensory proteins (CSPs). Tissue expression and phylogenetic tree analyses showed that one CYP (EgriCYP341-fragment3), one OBP (EgriOBP4), and one CSP (EgriCSP10) gene displayed an enriched expression in the PGs, and that EgriOBP2, 3, and 25 are clustered in the moth pheromone-binding protein clade. We considered these our candidate genes. Our results yielded large-scale PG sequence information for further functional studies" |
| Keywords: | "Animals Biological Transport Camellia sinensis/parasitology Computational Biology/methods Female *Gene Expression Profiling Gene Expression Regulation *Genes, Insect High-Throughput Nucleotide Sequencing Male Molecular Sequence Annotation Moths/classifica;" |
| Notes: | "MedlineLi, Zhao-Qun Ma, Long Yin, Qian Cai, Xiao-Ming Luo, Zong-Xiu Bian, Lei Xin, Zhao-Jun He, Peng Chen, Zong-Mao eng Research Support, Non-U.S. Gov't England 2018/01/11 G3 (Bethesda). 2018 Mar 2; 8(3):899-908. doi: 10.1534/g3.117.300543" |
