| Title: | "Effects of short-term exposure to volatile pesticide dichlorvos on the olfactory systems in Spodoptera litura: Calcium homeostasis, synaptic plasticity and apoptosis" |
| Author(s): | Li SS; Fang SM; Chen J; Zhang Z; Yu QY; |
| Address: | "School of Life Sciences, Chongqing University, Chongqing 400044, China. College of Life Science, China West Normal University, Nanchong 637002, Sichuan, China. School of Life Sciences, Chongqing University, Chongqing 400044, China. Electronic address: yuqy@cqu.edu.cn" |
| DOI: | 10.1016/j.scitotenv.2022.161050 |
| ISSN/ISBN: | 1879-1026 (Electronic) 0048-9697 (Linking) |
| Abstract: | "Volatile pesticides are a growing environmental and public health concern. However, little attention has been paid to its olfactory neurotoxic effect on pests and non-target organisms. Dichlorvos is a widely used organophosphorus fumigant that is ubiquitous in the environment. This study aims to explore the mode of action of the volatile dichlorvos-mediated olfactory impairment using a lepidopteran insect Spodoptera litura as a model. It was indicated that electroantennogram amplitudes of the male moths' response to sex pheromones and phenylacetaldehyde were reduced by approximately 20 % after 12-h fumigation exposure. RNA-Sequencing analysis revealed that down-regulation of trypsin and CLIC2 might be responsible for inhibition of odor recognition in the antenna, the peripheral olfactory tissue. In the head, 822 (84.05 %) of the 978 differentially expressed genes (DEGs) were up-regulated, of which seven DEGs encoding transcription factors may mainly modulate the stress-regulatory networks. Combining transcriptome with brain calcium imaging and Annexin V-mCherry staining experiments showed that volatile dichlorvos mainly disrupts Ca(2+) homeostasis and synaptic plasticity, induces apoptosis in the central nervous system, and further leads to olfactory dysfunction. Overall, this study highlighted a comprehensive work model for dichlorvos-induced olfactory impairment in S. litura and may provide insights into toxic effects of airborne organophosphates on non-target organisms" |
| Keywords: | Animals Male *Pesticides Spodoptera/genetics Dichlorvos/toxicity Calcium Neuronal Plasticity Apoptosis Larva/genetics Olfactory dysfunction Spodoptera litura Stress regulatory network Synaptic transmission Volatile dichlorvos; |
| Notes: | "MedlineLi, Shu-Shang Fang, Shou-Min Chen, Jie Zhang, Ze Yu, Quan-You eng Netherlands 2022/12/23 Sci Total Environ. 2023 Mar 15; 864:161050. doi: 10.1016/j.scitotenv.2022.161050. Epub 2022 Dec 20" |
