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« Previous AbstractMapping neural circuits with activity-dependent nuclear import of a transcription factor    Next AbstractDetermination of the release rate of aldehyde pheromones from insect lures by cold trapping and direct bioluminescence analysis »

J Chem Ecol


Title:Development of a bioluminescence assay for aldehyde pheromones of insects : I. Sensitivity and specificity
Author(s):Meighen EA; Slessor KN; Grant GG;
Address:"Department of Biochemistry, McGill University, 3655 Drummond Street, H3G 1Y6, Montreal, Quebec, Canada"
Journal Title:J Chem Ecol
Year:1982
Volume:8
Issue:6
Page Number:911 - 921
DOI: 10.1007/BF00987658
ISSN/ISBN:0098-0331 (Print) 0098-0331 (Linking)
Abstract:"A rapid, quantitative assay for long-chain aldehydes based on bacterial luminescence was developed. Significant luminescent responses were obtained with both saturated and unsaturated aldehydes of 12-18 carbons. Maximum responses were obtained with the 14- and 16-carbon compounds, including those that are known insect sex pheromones. The bioluminescent response was linearly related to the amount of aldehyde over a 10(4)-10(5) concentration range with as little as 0.1 pmol ( approximately 20 pg) of aldehyde being detected. The bioluminescent assay represents a new quantitative tool for rapidly measuring aldehyde pheromones of insects"
Keywords:
Notes:"PubMed-not-MEDLINEMeighen, E A Slessor, K N Grant, G G eng 1982/06/01 J Chem Ecol. 1982 Jun; 8(6):911-21. doi: 10.1007/BF00987658"

 
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