| Title: | Integrated Transcriptome and Metabolome Analysis to Identify Sugarcane Gene Defense against Fall Armyworm (Spodoptera frugiperda) Herbivory |
| Author(s): | Li AM; Wang M; Chen ZL; Qin CX; Liao F; Wu Z; He WZ; Lakshmanan P; Pan YQ; Huang DL; |
| Address: | "Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China. Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518000, China" |
| ISSN/ISBN: | 1422-0067 (Electronic) 1422-0067 (Linking) |
| Abstract: | "Sugarcane is the most important sugar crop, contributing >/=80% to total sugar production around the world. Spodoptera frugiperda is one of the main pests of sugarcane, potentially causing severe yield and sugar loss. The identification of key defense factors against S. frugiperda herbivory can provide targets for improving sugarcane resistance to insect pests by molecular breeding. In this work, we used one of the main sugarcane pests, S. frugiperda, as the tested insect to attack sugarcane. Integrated transcriptome and metabolomic analyses were performed to explore the changes in gene expression and metabolic processes that occurred in sugarcane leaf after continuous herbivory by S. frugiperda larvae for 72 h. The transcriptome analysis demonstrated that sugarcane pest herbivory enhanced several herbivory-induced responses, including carbohydrate metabolism, secondary metabolites and amino acid metabolism, plant hormone signaling transduction, pathogen responses, and transcription factors. Further metabolome analysis verified the inducement of specific metabolites of amino acids and secondary metabolites by insect herbivory. Finally, association analysis of the transcriptome and metabolome by the Pearson correlation coefficient method brought into focus the target defense genes against insect herbivory in sugarcane. These genes include amidase and lipoxygenase in amino acid metabolism, peroxidase in phenylpropanoid biosynthesis, and pathogenesis-related protein 1 in plant hormone signal transduction. A putative regulatory model was proposed to illustrate the sugarcane defense mechanism against insect attack. This work will accelerate the dissection of the mechanism underlying insect herbivory in sugarcane and provide targets for improving sugarcane variety resistance to insect herbivory by molecular breeding" |
| Keywords: | Animals Spodoptera/genetics *Herbivory *Saccharum/genetics Transcriptome Plant Growth Regulators Metabolome Insecta/physiology Edible Grain/genetics Sugars Amino Acids/genetics RNA sequencing Spodoptera frugiperda metabolism plant defense sugarcane; |
| Notes: | "MedlineLi, Ao-Mei Wang, Miao Chen, Zhong-Liang Qin, Cui-Xian Liao, Fen Wu, Zhen He, Wei-Zhong Lakshmanan, Prakash Pan, You-Qiang Huang, Dong-Liang eng 2020YFD1000600/the National Key R& D Program of China/ 2021GXNSFBA075010/the Natural Science Foundation of Guangxi/ AA20108005/the Science and Technology Major Project of Guangxi/ AA22036003/the Science and Technology Major Project of Guangxi/ CARS-170206/the earmarked fund for CARS/ 2021JM34/the Fundamental Research Fund of Guangxi Academy of Agriculture Sciences/ 2021YT010/the Fundamental Research Fund of Guangxi Academy of Agriculture Sciences/ Switzerland 2022/11/27 Int J Mol Sci. 2022 Nov 8; 23(22):13712. doi: 10.3390/ijms232213712" |
