| Title: | A microfluidic system for dynamic yeast cell imaging |
| Author(s): | Lee PJ; Helman NC; Lim WA; Hung PJ; |
| Address: | "CellASIC Corporation, San Leandro, CA 94577, USA. pjlee@cellasic.com" |
| ISSN/ISBN: | 0736-6205 (Print) 0736-6205 (Linking) |
| Abstract: | "The investigation of cellular processes and gene regulatory networks within living cells requires the development of improved technology for dynamic, single cell imaging. Here, we demonstrate a microfluidic system capable of mechanical trapping of yeast cells with continuous flow and flow switching capability during time-lapse high magnification fluorescence imaging. The novel functionality of the system was validated by observing the response of pheromone-induced expression of GFP in Saccharomyces cerevisiae" |
| Keywords: | Green Fluorescent Proteins/metabolism Membrane Proteins Microfluidics/*methods Recombinant Fusion Proteins/metabolism Saccharomyces cerevisiae/*cytology Saccharomyces cerevisiae Proteins/metabolism; |
| Notes: | "MedlineLee, Philip J Helman, Noah C Lim, Wendell A Hung, Paul J eng Research Support, Non-U.S. Gov't England 2008/02/08 Biotechniques. 2008 Jan; 44(1):91-5. doi: 10.2144/000112673" |
