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Gene

Title:		Molecular cloning and expression of Hedychium coronarium farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis of floral and wounding/herbivory induced leaf volatile sesquiterpenoids
Author(s):		Lan JB; Yu RC; Yu YY; Fan YP;
Address:		"Center of Flower Research, College of Horticulture, South China Agricultural University, Guangzhou 510642, China"
Journal Title:		Gene
Year:		2013
Volume:		20130117
Issue:		2
Page Number:		360 - 367
DOI:		10.1016/j.gene.2013.01.007
ISSN/ISBN:		1879-0038 (Electronic) 0378-1119 (Linking)
Abstract:		"Farnesyl pyrophosphate synthase (FPPS EC 2.5.1.10) catalyzes the production of farnesyl pyrophosphate (FPP), which is a key precursor for many sesquiterpenoids such as floral scent and defense volatiles against herbivore attack. Here we report a new full-length cDNA encoding farnesyl diphosphate synthase from Hedychium coronarium. The open reading frame for full-length HcFPPS encodes a protein of 356 amino acids, which is 1068 nucleotides long with calculated molecular mass of 40.7 kDa. Phylogenetic tree analysis indicates that HcFPPS belongs to the plant FPPS super-family and has strong relationship with FPPS from Musa acuminata. Expression of the HcFPPS gene in Escherichia coli yielded FPPS activity. Tissue-specific and developmental analyses of the HcFPPS mRNA and corresponding volatile sesquiterpenoid levels in H. coronarium flowers revealed that the HcFPPS might play a regulatory role in floral volatile sesquiterpenoid biosynthesis. The emission of the FPP-derived volatile terpenoid correlates with strong expression of HcFPPS induced by mechanical wounding and Udaspes folus-damage in leaves, which suggests that HcFPPS may have an important ecological function in H. coronarium vegetative organ"
Keywords:		"Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Complementary/genetics Flowers/genetics/metabolism *Gene Expression Regulation, Plant Geranyltranstransferase/*genetics/metabolism Herbivory Molecular Sequence Data Phylogeny Plant Leaves/genetics/;"
Notes:		"MedlineLan, Jian-bin Yu, Rang-cai Yu, Yun-yi Fan, Yan-ping eng Research Support, Non-U.S. Gov't Netherlands 2013/01/22 Gene. 2013 Apr 15; 518(2):360-7. doi: 10.1016/j.gene.2013.01.007. Epub 2013 Jan 17"