| Title: | Comparing passive dosing and solvent spiking methods to determine the acute toxic effect of pentachlorophenol on Daphnia magna |
| Author(s): | Kwon HA; Jeong Y; Jeon HP; Kim S; |
| Address: | "Environmental Safety Group, KIST Europe, Korea Institute of Science and Technology, Campus E7.1, 66123, Saarbrucken, Germany. Division of Energy & Environment Technology, University of Science and Technology, Daejeon, 34113, Korea. Institute for Environmental Research, RWTH Aachen University, Worringerweg 1, 52076, Aachen, Germany. Department of Land, Water and Environment Research, Korea Institute of Civil Engineering and Building Technology, Daehwa-Dong 283, Goyangdae-Ro, Ilsanseo-Gu, Goyang-Si, Gyeonggi-Do, 10223, Korea. Environmental Safety Group, KIST Europe, Korea Institute of Science and Technology, Campus E7.1, 66123, Saarbrucken, Germany. fatherofdamin@ks.ac.kr. Division of Energy & Environment Technology, University of Science and Technology, Daejeon, 34113, Korea. fatherofdamin@ks.ac.kr. Department of Pharmaceutical Science and Technology, Kyungsung University, 309, Suyeong-ro, Nam-gu, Busan, 48434, Korea. fatherofdamin@ks.ac.kr" |
| DOI: | 10.1007/s10646-020-02172-w |
| ISSN/ISBN: | 1573-3017 (Electronic) 0963-9292 (Linking) |
| Abstract: | "Pentachlorophenol (PCP) is a widespread and persistent hydrophobic organic pollutant in the environment despite its restricted public use. Risk assessment of such hydrophobic organic compounds (HOCs) is challenging because sorption and volatilization issues during toxicity test often lead to inconsistent exposure concentration. Considering the hydrophobicity of the PCP, in this study, a passive dosing format was applied by adopting a silicone O-ring as a reservoir and evaluated its applicability on the determination of PCP on Daphnia magna. Results obtained with passive dosing method were compared with that of solvent spiking method. We hypothesized that the passive dosing method may provide more reliable and accurate toxicity results than conventional solvent spiking approach. As a result, the partition coefficient of PCP between methanol and a test medium (log K(MeOH:ISO)) was 2.1, which enabled the maintenance of reliable exposure concentration throughout the experiment. In the acute toxicity tests, passive dosing and solvent spiking showed similar EC50 values of 576 and 485 microg/L for 24 h, and 362 and 374 microg/L for 48 h, respectively, which overlap with EC50 values of previous studies. Altogether, both methods were suitable for the acute toxicity assessment of hydrophobic PCP" |
| Keywords: | "Animals Daphnia/*physiology Dose-Response Relationship, Drug Environmental Pollutants Hydrophobic and Hydrophilic Interactions Pentachlorophenol/*toxicity Risk Assessment Solvents Toxicity Tests, Acute Water Pollutants, Chemical/*toxicity Daphnia magna Ec;" |
| Notes: | "MedlineKwon, Hyun-Ah Jeong, Yoonah Jeon, Hyun Pyo Kim, Sanghun eng 11896/Korea Institute of Science and Technology Europe/ 2020/03/04 Ecotoxicology. 2020 Apr; 29(3):286-294. doi: 10.1007/s10646-020-02172-w. Epub 2020 Mar 2" |
