| Title: | Detection and validation of volatile metabolic patterns over different strains of two human pathogenic bacteria during their growth in a complex medium using multi-capillary column-ion mobility spectrometry (MCC-IMS) |
| Author(s): | Kunze N; Gopel J; Kuhns M; Junger M; Quintel M; Perl T; |
| Address: | "Department of Anaesthesiology, Emergency and Intensive Care Medicine, University of Gottingen, Robert-Koch-Strasse 40, 37075 Gottingen, Germany. nkunze@gwdg.de" |
| Journal Title: | Appl Microbiol Biotechnol |
| DOI: | 10.1007/s00253-013-4762-8 |
| ISSN/ISBN: | 1432-0614 (Electronic) 0175-7598 (Print) 0175-7598 (Linking) |
| Abstract: | "Headspace analyses over microbial cultures using multi-capillary column-ion mobility spectrometry (MCC-IMS) could lead to a faster, safe and cost-effective method for the identification of pathogens. Recent studies have shown that MCC-IMS allows identification of bacteria and fungi, but no information is available from when on during their growth a differentiation between bacteria is possible. Therefore, we analysed the headspace over human pathogenic reference strains of Escherichia coli and Pseudomonas aeruginosa at four time points during their growth in a complex fluid medium. In order to validate our findings and to answer the question if the results of one bacterial strain can be transferred to other strains of the same species, we also analysed the headspace over cultures from isolates of random clinical origin. We detected 19 different volatile organic compounds (VOCs) that appeared or changed their signal intensity during bacterial growth. These included six VOCs exclusively changing over E. coli cultures and seven exclusively changing over P. aeruginosa cultures. Most changes occurred in the late logarithmic or static growth phases. We did not find differences in timing or trends in signal intensity between VOC patterns of different strains of one species. Our results show that differentiation of human pathogenic bacteria by headspace analyses using MCC-IMS technology is best possible during the late phases of bacterial growth. Our findings also show that VOC patterns of a bacterial strain can be transferred to other strains of the same species" |
| Keywords: | Bacteriological Techniques/*methods Escherichia coli/*classification/growth & development/*metabolism Humans Pseudomonas aeruginosa/*classification/growth & development/*metabolism Spectrum Analysis/*methods Volatile Organic Compounds/*analysis; |
| Notes: | "MedlineKunze, Nils Gopel, Julia Kuhns, Martin Junger, Melanie Quintel, Michael Perl, Thorsten eng Evaluation Study Research Support, Non-U.S. Gov't Germany 2013/03/08 Appl Microbiol Biotechnol. 2013 Apr; 97(8):3665-76. doi: 10.1007/s00253-013-4762-8. Epub 2013 Mar 7" |
