Title: | Effects of Aspergillus species inoculation and their enzymatic activities on the formation of volatile components in fermented soybean paste (doenjang) |
Author(s): | Kum SJ; Yang SO; Lee SM; Chang PS; Choi YH; Lee JJ; Hurh BS; Kim YS; |
Address: | "Department of Food Science and Engineering, Ewha Womans University , 11-1 Daehyun-dong, Seodaemun-gu, Seoul 120-750, Republic of Korea" |
ISSN/ISBN: | 1520-5118 (Electronic) 0021-8561 (Linking) |
Abstract: | "The volatile components of rice-koji doenjang produced in association with various Aspergillus species (A. oryzae, A. sojae, and A. kawachii) during 8 weeks of fermentation were compared using gas chromatography-mass spectrometry analysis and multivariate statistical analysis. In addition, the activities of diverse enzymes (alpha-amylase, protease, lipase, and esterase) were determined to investigate their effects on the formation of volatile compounds. Regardless of the fungi types, carbonyls including 4-methylheptan-2-one, heptan-2-one, (E)-hept-2-enal, and hexanal were found to contribute mainly to early phase fermentation, whereas the contents of ethyl esters of long-chained fatty acids were considerably enhanced in the latter stage of fermentation. With the exception of alpha-amylase, the activities of the studied enzymes generally increased as fermentation proceeded, and there were significant differences in enzymes activities between the species of fungi. The lipase activity was strongly correlated with the formation of long-chain fatty acid esters, which could be related to the distinctive organoleptic properties of rice-koji doenjang" |
Keywords: | Aspergillus/enzymology/*metabolism Esterases/metabolism Fermentation Fungal Proteins/*metabolism Gas Chromatography-Mass Spectrometry Lipase/metabolism Peptide Hydrolases/metabolism Soy Foods/analysis/*microbiology Soybeans/chemistry/*microbiology Volatil; |
Notes: | "MedlineKum, Sun-Joo Yang, Seung-Ok Lee, Sang Mi Chang, Pahn-Shick Choi, Yong Ho Lee, Jae Jung Hurh, Byung Serk Kim, Young-Suk eng Research Support, Non-U.S. Gov't 2015/01/16 J Agric Food Chem. 2015 Feb 11; 63(5):1401-18. doi: 10.1021/jf5056002. Epub 2015 Jan 28" |