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ACS Chem Biol

Title:		Metabolite induction of Caenorhabditis elegans dauer larvae arises via transport in the pharynx
Author(s):		Baiga TJ; Guo H; Xing Y; O'Doherty GA; Dillin A; Austin MB; Noel JP; La Clair JJ;
Address:		"Howard Hughes Medical Institute, Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 10010 North Torrey Pines Road, La Jolla, California 92037, USA"
Journal Title:		ACS Chem Biol
Year:		2008
Volume:		20080401
Issue:		5
Page Number:		294 - 304
DOI:		10.1021/cb700269e
ISSN/ISBN:		1554-8937 (Electronic) 1554-8929 (Print) 1554-8929 (Linking)
Abstract:		"Caenorhabditis elegans sense natural chemicals in their environment and use them as cues to regulate their development. This investigation probes the mechanism of sensory trafficking by evaluating the processing of fluorescent derivatives of natural products in C. elegans. Fluorescent analogs of daumone, an ascaroside, and apigenin were prepared by total synthesis and evaluated for their ability to induce entry into a nonaging dauer state. Fluorescent imaging detailed the uptake and localization of every labeled compound at each stage of the C. elegans life cycle. Comparative analyses against natural products that did not induce dauer indicated that dauer-triggering natural products accumulated in the cuticle of the pharnyx. Subsequent transport of these molecules to amphid neurons signaled entry into the dauer state. These studies provide cogent evidence supporting the roles of the glycosylated fatty acid daumone and related ascarosides and the ubiquitous plant flavone apigenin as chemical cues regulating C. elegans development"
Keywords:		Animals Biological Transport *Caenorhabditis elegans/drug effects/growth & development/metabolism *Fatty Acids/chemical synthesis/chemistry/pharmacology *Fluorescent Dyes/chemical synthesis/chemistry/pharmacology Larva/drug effects/growth & development/me;
Notes:		"MedlineBaiga, Thomas J Guo, Haibing Xing, Yalan O'Doherty, George A Dillin, Andrew Austin, Michael B Noel, Joseph P La Clair, James J eng HHMI/Howard Hughes Medical Institute/ R01 GM063150/GM/NIGMS NIH HHS/ HL-24736/HL/NHLBI NIH HHS/ Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. 2008/04/02 ACS Chem Biol. 2008 May 16; 3(5):294-304. doi: 10.1021/cb700269e. Epub 2008 Apr 1"