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J Chem Ecol

Title:		"Identification of components of the female sex pheromone of the Simao pine caterpillar moth, Dendrolimus kikuchii Matsumura"
Author(s):		Kong XB; Sun XL; Wang HB; Zhang Z; Zhao CH; Booij KC;
Address:		"Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Key Laboratory of Forest Protection, State Forestry Administration, Beijing, 100091, China"
Journal Title:		J Chem Ecol
Year:		2011
Volume:		20110317
Issue:		4
Page Number:		412 - 419
DOI:		10.1007/s10886-011-9932-5
ISSN/ISBN:		1573-1561 (Electronic) 0098-0331 (Linking)
Abstract:		"The pine caterpillar moth, Dendrolimus kikuchii Matsumura (Lepidoptera: Lasiocampidae), is a pest of economic importance on pine in southwest China. Three active compounds were detected during analyses of solvent extracts and effluvia sampled by solid phase microextraction (SPME) from virgin female D. kikuchii using gas chromatography (GC) coupled with electroantennographic (EAG) recording with antennae from a male moth. The compounds were identified as (5Z,7E)-5,7-dodecadien-1-yl acetate (Z5,E7-12:OAc), (5Z,7E)-5,7-dodecadien-1-ol (Z5,E7-12:OH), and (5Z)-5-dodecenyl acetate (Z5-12:OAc) by comparison of their GC retention indices, mass spectra, and EAG activities with those of synthetic standards. Microchemical reactions of gland extracts provided further information confirming the identifications of the three components. Solvent extractions and SPME samples of pheromone effluvia from virgin calling females provided 100:18:0.6 and 100:7:1 ratios of Z5,E7-12:OAc:Z5,E7-12:OH:Z5-12:OAc, respectively. Field behavioral assays showed that Z5,E7-12:OAc and Z5,E7-12:OH were essential for attraction of male D. kikuchii moths. However, the most attractive blend contained these three components in a 100:20:25 ratio in a gray rubber septa. Our results demonstrated that the blend of Z5,E7-12:OAc, Z5,E7-12:OH, and Z5-12:OAc comprise the sex pheromone of D. kikuchii. The optimized three-component lure blend is recommended for monitoring D. kikuchii infestations"
Keywords:		"Acetates/*chemistry/*isolation & purification Animals Chromatography, Gas Fatty Alcohols/*chemistry/*isolation & purification Female Male Mass Spectrometry *Moths/anatomy & histology/chemistry *Sex Attractants/analysis/chemistry Solid Phase Microextractio;"
Notes:		"MedlineKong, Xiang-Bo Sun, Xiao-Ling Wang, Hong-Bin Zhang, Zhen Zhao, Cheng-Hua Booij, Kees C J H eng Research Support, Non-U.S. Gov't 2011/03/19 J Chem Ecol. 2011 Apr; 37(4):412-9. doi: 10.1007/s10886-011-9932-5. Epub 2011 Mar 17"