| Title: | Chitosan oligosaccharides affect xanthone and VOC biosynthesis in Hypericum perforatum root cultures and enhance the antifungal activity of root extracts |
| Author(s): | Badiali C; De Angelis G; Simonetti G; Brasili E; Tobaruela EC; Purgatto E; Yin H; Valletta A; Pasqua G; |
| Address: | "Department of Environmental Biology, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy. Department of Public Health and Infectious Diseases, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy. Department of Food Sciences and Experimental Nutrition/FORC-Food Research Center, University of Sao Paulo, Av. Prof. Lineu Prestes 580, Sao Paulo, 05508-000, Brazil. Liaoning Provincial Key Laboratory of Carbohydrates, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, People's Republic of China. Department of Environmental Biology, Sapienza University of Rome, Piazzale Aldo Moro 5, 00185, Rome, Italy. alessio.valletta@uniroma1.it" |
| DOI: | 10.1007/s00299-018-2317-2 |
| ISSN/ISBN: | 1432-203X (Electronic) 0721-7714 (Linking) |
| Abstract: | "Water-soluble chitosan oligosaccharides (COS) affect xanthone and volatile organic compound content, as well as antifungal activity against human pathogenic fungi of extracts obtained from Hypericum perforatum root cultures. Several studies have demonstrated the elicitor power of chitosan on xanthone biosynthesis in root cultures of H. perforatum. One of the major limitations to the use of chitosan, both for basic and applied research, is the need to use acidified water for solubilization. To overcome this problem, the elicitor effect of water-soluble COS on the biosynthesis of both xanthones and volatile organic compounds (VOCs) was evaluated in the present study. The analysis of xanthones and VOCs was performed by HPLC and GC-MS headspace analysis. The obtained results showed that COS are very effective in enhancing xanthone biosynthesis. With 400 mg L(-1) COS, a xanthone content of about 30 mg g(-1) DW was obtained. The antifungal activity of extracts obtained with 400 mg L(-1) COS was the highest, with MIC(50) of 32 microg mL(-1) against Candida albicans and 32-64 microg mL(-1) against dermatophytes, depending on the microorganism. Histochemical investigations suggested the accumulation of isoprenoids in the secretory ducts of H. perforatum roots. The presence of monoterpenes and sesquiterpenes was confirmed by the headspace analysis. Other volatile hydrocarbons have been identified. The biosynthesis of most VOCs showed significant changes in response to COS, suggesting their involvement in plant-fungus interactions" |
| Keywords: | Antifungal Agents/*pharmacology Candida albicans/drug effects Chitosan/*pharmacology Hypericum/*chemistry Oligosaccharides/pharmacology Plant Extracts/*pharmacology Plant Oils/*metabolism Plant Roots/drug effects Volatile Organic Compounds/*metabolism Xan; |
| Notes: | "MedlineBadiali, Camilla De Angelis, Giulia Simonetti, Giovanna Brasili, Elisa Tobaruela, Eric de Castro Purgatto, Eduardo Yin, Heng Valletta, Alessio Pasqua, Gabriella eng Germany 2018/06/30 Plant Cell Rep. 2018 Nov; 37(11):1471-1484. doi: 10.1007/s00299-018-2317-2. Epub 2018 Jun 28" |
