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Plants (Basel)

Title:		Cloning and Expression of a Perilla frutescens Cytochrome P450 Enzyme Catalyzing the Hydroxylation of Phenylpropenes
Author(s):		Baba M; Yamada KI; Ito M;
Address:		"Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Kyoto 606-8501, Japan. Department of Pharmaceutical Organic Chemistry, Graduate School of Pharmaceutical Sciences, Tokushima University, Shomachi, Tokushima 770-8505, Japan"
Journal Title:		Plants (Basel)
Year:		2020
Volume:		20200501
Issue:		5
Page Number:		-
DOI:		10.3390/plants9050577
ISSN/ISBN:		2223-7747 (Print) 2223-7747 (Electronic) 2223-7747 (Linking)
Abstract:		"Phenylpropanoid volatile components in plants are useful and valuable not only as flavorings, but also as medicines and food supplements. The pharmacological actions and toxicities of these compounds have been well studied but their synthetic pathways are generally unclear. In this study, we mined expressed sequence tag libraries of pure strains of perilla maintained for over 30 years for their oil type and conducted gas chromatography-mass spectrometry analyses of the perilla oils to confirm the presence of monohydrates speculated to be intermediates of the phenylpropene synthetics pathways. These putative monohydrate intermediates and their regioisomers were synthesized to identify the reaction products of assays of heterologously expressed enzymes. An enzyme involved in the synthesis of a phenylpropanoid volatile component was identified in perilla. Expression of this enzyme in Saccharomyces cerevisiae showed that it is a member of the cytochrome P450 family and catalyzes the introduction of a hydroxy group onto myristicin to form an intermediate of dillapiole. The enzyme had high sequence similarity to a CYP71D family enzyme, high regiospecificity, and low substrate specificity. This study may aid the elucidation of generally unexploited biosynthetic pathways of phenylpropanoid volatile components"
Keywords:		biosynthetic pathway cytochrome P450 molecular cloning perilla phenylpropanoid volatile compound;
Notes:		"PubMed-not-MEDLINEBaba, Mariko Yamada, Ken-Ichi Ito, Michiho eng Switzerland 2020/05/07 Plants (Basel). 2020 May 1; 9(5):577. doi: 10.3390/plants9050577"