| Title: | Development of a direct exposure system for studying the mechanisms of central neurotoxicity caused by volatile organic compounds |
| Author(s): | Kanemitsu M; Fueta Y; Ishidao T; Aou S; Hori H; |
| Address: | "Department of Environmental Management and Control, School of Health Sciences, University of Occupational and Environmental Health, Japan" |
| DOI: | 10.2486/indhealth.2015-0076 |
| ISSN/ISBN: | 1880-8026 (Electronic) 0019-8366 (Print) 0019-8366 (Linking) |
| Abstract: | "Many volatile organic compounds (VOCs) used in work places are neurotoxic. However, it has been difficult to study the cellular mechanisms induced by a direct exposure to neurons because of their high volatility. The objective of this study was to establish a stable system for exposing brain slices to VOCs. With a conventional recording system for brain slices, it is not possible to keep a constant bath concentration of relatively highly volatile solvents, e.g. 1-bromopropane (1-BP). Here we report a new exposure system for VOCs that we developed in which a high concentration of oxygen is dissolved to a perfused medium applying a gas-liquid equilibrium, and in which the tubing is made of Teflon, non adsorptive material. Using our system, the bath concentration of the perfused 1-BP remained stable for at least 2 h in the slice chamber. Both 6.4 and 2.2 mM of 1-BP did not change the paired-pulse response, but fully suppressed long-term potentiation in the dentate gyrus (DG) of hippocampal slices obtained from rats, suggesting that 1-BP decreases synaptic plasticity in the DG at the concentrations tested. Our new system can be applicable for investigating the underlying mechanisms of the neurotoxicity of VOCs at the cellular level" |
| Keywords: | "Animals Dentate Gyrus/*drug effects Diffusion Chambers, Culture Hydrocarbons, Brominated/analysis/toxicity Male Neuronal Plasticity/drug effects Phase Transition Rats Synapses/*drug effects Tissue Culture Techniques Volatile Organic Compounds/analysis/*to;" |
| Notes: | "MedlineKanemitsu, Masanari Fueta, Yukiko Ishidao, Toru Aou, Shuji Hori, Hajime eng Research Support, Non-U.S. Gov't Japan 2015/09/01 Ind Health. 2016; 54(1):42-9. doi: 10.2486/indhealth.2015-0076. Epub 2015 Aug 28" |
