| Title: | Co-liquefaction with acetone and GC analysis of volatile compounds in exhaled breath as lung cancer biomarkers |
| Author(s): | Jouyban A; Djozan D; Mohammadandashti P; Alizadeh-Nabil A; Ghorbanpour H; Khoubnasabjafari M; Mohammadzadeh M; |
| Address: | "Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran. Kimia Idea Pardaz Azarbayjan (KIPA) Science Based Company, Tabriz University of Medical Sciences, Tabriz, Iran. Department of Chemistry, College of Sciences, East Azarbayjan Sciences and Research Branch, Islamic Azad University, Tabriz, Iran. Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Tuberculosis and Lung Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Department of Radiotherapy, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran" |
| ISSN/ISBN: | 2228-5652 (Print) 2228-5660 (Electronic) 2228-5652 (Linking) |
| Abstract: | "Introduction: A simple, rapid and low cost method for enrichment of volatile organic compounds (VOCs) from exhaled breath (EB) is presented. Methods: A 1000 mL home-made extraction device was filled with EB. The VOCs were extracted and condensed in 0.5 mL acetone. Recognition of volatiles in the real studied EB samples was performed by a GC-MS. Results: The method displays an extraction efficiency of >86% with the enrichment factor of 1929 for octanal. Limits of detection and quantification, and linear dynamic range were 0.008, 0.026 and 0.026-400 ng/mL respectively. Analysis of real samples showed the existence of more than 100 compounds in EB of healthy volunteers and patients with lung cancer before and after treatment. Exhaled octanal concentration was significantly higher in lung cancer patient than in healthy volunteers and lung cancer patient after treatment. Conclusion: Having used the proposed approach, high extraction recovery (up to 86%) was attained for the lung cancer marker, octanal, as an important biomarker. Our findings on smaples of EB of healthy controls and patients with lung cancer before and after treatment provide complelling evidence upon the effectiveness of the developed method" |
| Keywords: | Cancer biomarkers Co-liquefaction Exhaled breath Lung cancer Volatile compounds; |
| Notes: | "PubMed-not-MEDLINEJouyban, Abolghasem Djozan, Djavanshir Mohammadandashti, Parastou Alizadeh-Nabil, Aliakbar Ghorbanpour, Hooshangh Khoubnasabjafari, Maryam Mohammadzadeh, Mohammad eng Iran 2017/07/29 Bioimpacts. 2017; 7(2):99-108. doi: 10.15171/bi.2017.13. Epub 2017 Jun 20" |
